CAJ | 학술논문

目的：建立基于芯片点样技术的荧光定量PCR（RT‐PCR）平台并将其用于HIV‐1和HCVRNA载量的检测。方法选择HIV‐1和HCV的保守区域设计引物，制备基因芯片管，建立RT‐PCR测定HIV‐1和HCV的反应体系，利用溶解曲线区分病毒种类。对该方法的灵敏度和特异度进行评价，并用临床标本验证其检测性能。结果该方法的特异度较好，其检测HCV、HIV‐1的最低检测限为1×103copy/mL，对RNA载量为1×103～1×106copy/mL的临床标本能准确检测。结论该方法为检测HCV、HIV‐1提供了新思路。
목적：건립기우심편점양기술적형광정량PCR（RT‐PCR）평태병장기용우HIV‐1화HCVRNA재량적검측。방법선택HIV‐1화HCV적보수구역설계인물，제비기인심편관，건립RT‐PCR측정HIV‐1화HCV적반응체계，이용용해곡선구분병독충류。대해방법적령민도화특이도진행평개，병용림상표본험증기검측성능。결과해방법적특이도교호，기검측HCV、HIV‐1적최저검측한위1×103copy/mL，대RNA재량위1×103～1×106copy/mL적림상표본능준학검측。결론해방법위검측HCV、HIV‐1제공료신사로。
Objective To establish the chip technology‐based real‐time PCR (RT‐PCR) platform and to apply it in the viral loads detection of HIV‐1 and HCV .Methods Based on the primers designed to aim at the conversed regions of HIV‐1 and HCV , The gene chip tube was prepared ,and the RT‐PCR reaction system was established for the simultaneous determination of viral loads .And the melting curves were used to distinguish viral species .The sensitivity and specificity of the method were estimated , and performance of the method was verified by using clinical samples .Results The specificity of the method was good .The lowest detectable limit of the detection method of HCV and HIV‐1 was 1 × 103 copy/mL .The clinical samples with viral loads around 1 × 103 -1 × 106 copy/mL could be detected accurately .Conclusion The method provides a new idea for the detection of HCV and HIV‐1 .