CAJ | 학술논문

目的：应用蛋白质组学技术筛选过敏性鼻炎（AR）患者鼻腔灌洗液中的蛋白标记物。方法采集10例AR患者及20例健康对照者过敏原刺激前后的鼻腔灌洗液，用双向电泳检测其中的蛋白组成，筛选差异表达的蛋白进行质谱分析鉴定。结果共筛选出6种差异表达的蛋白，在过敏原刺激后，乳铁蛋白、载脂蛋白A1（APOA1）及α1‐抗胰蛋白酶表达上调，PLUNC蛋白、结合珠蛋白、IgJ表达下调。结论筛选出的6种蛋白可能成为AR的生物标记物。
목적：응용단백질조학기술사선과민성비염（AR）환자비강관세액중적단백표기물。방법채집10례AR환자급20례건강대조자과민원자격전후적비강관세액，용쌍향전영검측기중적단백조성，사선차이표체적단백진행질보분석감정。결과공사선출6충차이표체적단백，재과민원자격후，유철단백、재지단백A1（APOA1）급α1‐항이단백매표체상조，PLUNC단백、결합주단백、IgJ표체하조。결론사선출적6충단백가능성위AR적생물표기물。
Objective To screen the biomarkers in nasal lavage fluid of patients with allergic rhinitis (AR) by proteomics tech‐nology .Methods The nasal lavage fluid samples of 10 cases of AR patients and 20 cases of normal controls were collected both be‐fore and after allergen stimulation tests .Two‐dimensioned gel eletrophoresis was used to detect the proteins in nasal lavage fluid samples .The proteins that showed different expression levels were identified by MALDI‐TOF/TOF mass spectrometry .Results Six kinds of proteins differentially expressed were screened out .The expressions of lactoferrin ,α1‐antitrypsin ,and APOA1 increased in the AR patients after being stimulated by allergens .On the contrary ,the expressions of PLUNC protein ,haptoglobin and IgJ de‐creased .Conclusion The six kinds of proteins might be the biomarkers of AR .