CAJ | 학술논문

目的：比较自然人群女性宫颈正常细胞、宫颈上皮癌前病变（CINⅢ级）及宫颈癌组织中HPV的基因型别分布情况。方法采用基因扩增结合基因芯片技术对1047例自然人群女性宫颈正常细胞（正常组）、173例宫颈上皮癌前病变（癌前病变组）及133例宫颈癌（宫颈癌组）组织标本进行23种HPV基因分型检测。结果正常组、癌前病变组、宫颈癌组分别检出HPV感染者109、159、121例，总HPV感染率分别为10．41％（109/1047）、91．91％（159/173）、90．98％（121/133）。结论基因扩增结合基因芯片检测技术可应用于宫颈细胞和组织标本的HPV基因分型检测。
목적：비교자연인군녀성궁경정상세포、궁경상피암전병변（CINⅢ급）급궁경암조직중HPV적기인형별분포정황。방법채용기인확증결합기인심편기술대1047례자연인군녀성궁경정상세포（정상조）、173례궁경상피암전병변（암전병변조）급133례궁경암（궁경암조）조직표본진행23충HPV기인분형검측。결과정상조、암전병변조、궁경암조분별검출HPV감염자109、159、121례，총HPV감염솔분별위10．41％（109/1047）、91．91％（159/173）、90．98％（121/133）。결론기인확증결합기인심편검측기술가응용우궁경세포화조직표본적HPV기인분형검측。
Objective To compare the genotype distribution of HPV in cervical cells of natural crowd and tissues of cervical in‐traepithelial neoplasia(CINⅢ grade) and cervical carcinomas patients .Methods PCR and gene‐chip technology were utilized for the genotype detection of 23 kinds of HPV in cell specimens from 1 047 women of natural crowd (normal group) and tissue specimens from 173 cases of cervical intraepithelial neoplasia(precancerosis group) and 133 cases of patients with cervical carcinoma (cervical carcinoma group) .Results There were 109 ,159 and 121 cases of HPV positive specimens respectively in normal group ,precancer‐osis group and cervical carcinoma group ,and the HPV infection rates were 10 .41% (109/1 047) ,91 .91% (159/173) and 90 .98%(121/133) ,respectively .Conclusion PCR and gene‐chip technology can be used to detect HPV genotypes in cervical cells and cer‐vical tissues specimens .