CAJ | 학술논문

目的：探讨反式白藜芦醇(TR,Trans-Resveratrol)对Aβ25-35损伤大鼠海马NMDA受体NR1、NR2A亚基表达的影响。方法选择经莫式水迷宫(MWM)训练合格的大鼠,随机分为6组：假手术组；模型组；TR高剂量组、TR中剂量组、TR低剂量组、安理申对照组。模型组通过在大鼠海马内注射Aβ25-35制模获得,在模型组基础上分别用TR高、中、低剂量TR干预获得TR高剂量组、TR中剂量组、TR低剂量组,同时用安理申干预获得安理申对照组,每组6只,采用实时聚合酶链反应法(real time PCR)检测6组大鼠海马NMDA受体NR1、NR2A表达。结果在MWM中模型组逃避潜伏期比TR高、中、低剂量组、安理申组和假手术组明显延长,各组NMDA受体NR1表达分别为(0.14±0.04),(1.51±0.54),(0.22±0.06),(0.37±0.08,0.61)±0.15)(LOW),(0.97±0.16)(安理申)。各组NMDA受体NR2A表达分别为(0.12±0.07)、(1.72±0.34)、(0.16±0.06)、(0.21±0.08)、(0.52±0.15)、(0.67±0.16)。结论TR可能有下调海马NMDAR1、NMDAR2A受体表达,从而减少海马细胞凋亡的作用。
목적：탐토반식백려호순(TR,Trans-Resveratrol)대Aβ25-35손상대서해마NMDA수체NR1、NR2A아기표체적영향。방법선택경막식수미궁(MWM)훈련합격적대서,수궤분위6조：가수술조；모형조；TR고제량조、TR중제량조、TR저제량조、안리신대조조。모형조통과재대서해마내주사Aβ25-35제모획득,재모형조기출상분별용TR고、중、저제량TR간예획득TR고제량조、TR중제량조、TR저제량조,동시용안리신간예획득안리신대조조,매조6지,채용실시취합매련반응법(real time PCR)검측6조대서해마NMDA수체NR1、NR2A표체。결과재MWM중모형조도피잠복기비TR고、중、저제량조、안리신조화가수술조명현연장,각조NMDA수체NR1표체분별위(0.14±0.04),(1.51±0.54),(0.22±0.06),(0.37±0.08,0.61)±0.15)(LOW),(0.97±0.16)(안리신)。각조NMDA수체NR2A표체분별위(0.12±0.07)、(1.72±0.34)、(0.16±0.06)、(0.21±0.08)、(0.52±0.15)、(0.67±0.16)。결론TR가능유하조해마NMDAR1、NMDAR2A수체표체,종이감소해마세포조망적작용。
Objective To observe the effects of trans-resveratrol (TR) on the expression of NMDA receptor NR1 and NR2A sub-units in hippocampal Aβ25-35 injured rat. Methods After being trained using Morris water maze (MWM), the qualified rats were selected and randomly divided into 6 groups: sham-operated group, model group, TR high dose group, TR middle dose group and TR low dose group, Donepezil group. The model group was established by injecting Aβ25-35 to hippocampus of the rats. After the model was established, it was treated with TR of high dose, moderate dose and low dose to make TR high, middle and low dose group, and Donepezil to make Donepezil group. Each group has 6 rats. Real time PCR was used to detect the mRNA expression of inducible nitric oxide synthase NMDAR1, NMDA R2A in hippocampus of the 6 groups of rats. Results The escape latency of model group was significantly longer than that of TR high, middle, low dose group, Donepezil group and sham-operated group in MWM. The mRNA expression of NR1 in each group was 0.14 ± 0.04, 1.51 ± 0.54, 0.22 ± 0.06, 0.37± 0.08, 0.61 ± 0.15(LOW), 0.97±0.16 (Donepezil), respectively, and the mRNA expression of NR2A was 0.12±0.07, 1.72±0.34, 0.16±0.06, 0.21±0.08, 0.52±0.15, 0.67±0.16, respectively. Conclusion TR may downregulate the mRNA expression of NMDA R1 and NMDAR2A in hip-pocampus, thereby reducing the cell apoptosis in hippocampus.