植物保护
植物保護
식물보호
PLANT PROTECTION
2014年
6期
135-139
,共5页
田兆丰%刘伟成%张涛涛%董丹%罗晨
田兆豐%劉偉成%張濤濤%董丹%囉晨
전조봉%류위성%장도도%동단%라신
番茄%病毒病%鉴定
番茄%病毒病%鑒定
번가%병독병%감정
tomato%virus disease%identification
近年来,番茄病毒病,特别是番茄黄化曲叶病毒(TYLCV)病在北京地区空前暴发,给番茄生产造成严重威胁,使番茄的产量和品质显著降低。20122013年在北京周边7个区县,采集疑似感染病毒的番茄植株样品325份,分别针对TYLCV、烟草花叶病毒(TMV)、黄瓜花叶病毒(CMV)3种病毒进行了PCR或ELISA 检测。检测结果表明,目前在北京地区大棚或温室内发生的番茄病毒病以TYLCV为主;露地番茄病毒复合侵染现象比较普遍,病毒检出率100%,其中TYLCV检出率达到75%以上。在TYLCV侵染的样品中,TYLCV和CMV复合侵染占20%左右,TYLCV和TMV复合侵染占15%左右。部分样品检测到TYLCV、CMV和TMV 3种病毒复合侵染的现象。
近年來,番茄病毒病,特彆是番茄黃化麯葉病毒(TYLCV)病在北京地區空前暴髮,給番茄生產造成嚴重威脅,使番茄的產量和品質顯著降低。20122013年在北京週邊7箇區縣,採集疑似感染病毒的番茄植株樣品325份,分彆針對TYLCV、煙草花葉病毒(TMV)、黃瓜花葉病毒(CMV)3種病毒進行瞭PCR或ELISA 檢測。檢測結果錶明,目前在北京地區大棚或溫室內髮生的番茄病毒病以TYLCV為主;露地番茄病毒複閤侵染現象比較普遍,病毒檢齣率100%,其中TYLCV檢齣率達到75%以上。在TYLCV侵染的樣品中,TYLCV和CMV複閤侵染佔20%左右,TYLCV和TMV複閤侵染佔15%左右。部分樣品檢測到TYLCV、CMV和TMV 3種病毒複閤侵染的現象。
근년래,번가병독병,특별시번가황화곡협병독(TYLCV)병재북경지구공전폭발,급번가생산조성엄중위협,사번가적산량화품질현저강저。20122013년재북경주변7개구현,채집의사감염병독적번가식주양품325빈,분별침대TYLCV、연초화협병독(TMV)、황과화협병독(CMV)3충병독진행료PCR혹ELISA 검측。검측결과표명,목전재북경지구대붕혹온실내발생적번가병독병이TYLCV위주;로지번가병독복합침염현상비교보편,병독검출솔100%,기중TYLCV검출솔체도75%이상。재TYLCV침염적양품중,TYLCV화CMV복합침염점20%좌우,TYLCV화TMV복합침염점15%좌우。부분양품검측도TYLCV、CMV화TMV 3충병독복합침염적현상。
In recent years,tomato virus disease,especially Tomato yellow leaf curl virus(TYLCV)disease in Bei-jing area occurred seriously,and the yield and quality of tomato reduced significantly. During 2012-2013,suspec-ted virus infection samples of tomato collected in seven districts around Beijing were detected by PCR or ELISA for TYLCV,Tobaccomosaicvirus(TMV)and Cucumbermosaicvirus(CMV),respectively. The results showed that TYLCV disease was dominant in the greenhouse. In the open field,the mixed infection of several virus was common,with the total detection rate up to 100% ,in which the positive rate for TYLCV was over 75% . In the TYLCV infected samples,the mixed infection for TYLCV and CMV accounted around 20% ,and about 15% for TYLCV and TMV. Some samples were found to be infected by the three viruses simultaneously.