植物保护
植物保護
식물보호
PLANT PROTECTION
2014年
6期
94-99
,共6页
金龟子绿僵菌%多菌灵%抗性水平%菌丝生长速率%产孢量
金龜子綠僵菌%多菌靈%抗性水平%菌絲生長速率%產孢量
금구자록강균%다균령%항성수평%균사생장속솔%산포량
Metarhizium anisopliae%carbendazim%resistance level%mycelia growth rate%sporulation
在室内条件下测定了供试绿僵菌对多菌灵的抗性。结果表明，供试菌株对多菌灵均表现为敏感，其中，Ma55菌株对多菌灵最敏感。采用分生孢子定向筛选的方法获得了金龟子绿僵菌抗多菌灵突变株，测定了抗性突变株对多菌灵的抗性差异。结果显示，在获得的抗性突变株中，MC-2的EC50最大，达到397．0643μg／mL，对多菌灵的抗性水平最高，抗性水平指数达到102．35。对Ma55抗多菌灵突变株的菌丝生长速率、产孢量及对棉花枯萎病菌和棉花红腐病菌的抑制效果的研究结果表明，抗性突变株的菌丝生长速率均比亲本菌株Ma55小，但产孢量均比Ma55大，其中，MC-2的产孢量最大，为5．12×106个／mL；MC-5次之，其产孢量为4．81×106个／mL，MC-8的产孢量在抗性突变株中最低。平板对峙培养结果显示，金龟子绿僵菌抗多菌灵突变株对棉花枯萎病菌和棉花红腐病菌菌丝生长的抑制作用均达到显著水平，其中，MC-2对棉花枯萎病菌、棉花红腐病菌菌丝生长的抑制作用最强，与亲本对照菌株Ma55差异不显著。
在室內條件下測定瞭供試綠僵菌對多菌靈的抗性。結果錶明，供試菌株對多菌靈均錶現為敏感，其中，Ma55菌株對多菌靈最敏感。採用分生孢子定嚮篩選的方法穫得瞭金龜子綠僵菌抗多菌靈突變株，測定瞭抗性突變株對多菌靈的抗性差異。結果顯示，在穫得的抗性突變株中，MC-2的EC50最大，達到397．0643μg／mL，對多菌靈的抗性水平最高，抗性水平指數達到102．35。對Ma55抗多菌靈突變株的菌絲生長速率、產孢量及對棉花枯萎病菌和棉花紅腐病菌的抑製效果的研究結果錶明，抗性突變株的菌絲生長速率均比親本菌株Ma55小，但產孢量均比Ma55大，其中，MC-2的產孢量最大，為5．12×106箇／mL；MC-5次之，其產孢量為4．81×106箇／mL，MC-8的產孢量在抗性突變株中最低。平闆對峙培養結果顯示，金龜子綠僵菌抗多菌靈突變株對棉花枯萎病菌和棉花紅腐病菌菌絲生長的抑製作用均達到顯著水平，其中，MC-2對棉花枯萎病菌、棉花紅腐病菌菌絲生長的抑製作用最彊，與親本對照菌株Ma55差異不顯著。
재실내조건하측정료공시록강균대다균령적항성。결과표명，공시균주대다균령균표현위민감，기중，Ma55균주대다균령최민감。채용분생포자정향사선적방법획득료금구자록강균항다균령돌변주，측정료항성돌변주대다균령적항성차이。결과현시，재획득적항성돌변주중，MC-2적EC50최대，체도397．0643μg／mL，대다균령적항성수평최고，항성수평지수체도102．35。대Ma55항다균령돌변주적균사생장속솔、산포량급대면화고위병균화면화홍부병균적억제효과적연구결과표명，항성돌변주적균사생장속솔균비친본균주Ma55소，단산포량균비Ma55대，기중，MC-2적산포량최대，위5．12×106개／mL；MC-5차지，기산포량위4．81×106개／mL，MC-8적산포량재항성돌변주중최저。평판대치배양결과현시，금구자록강균항다균령돌변주대면화고위병균화면화홍부병균균사생장적억제작용균체도현저수평，기중，MC-2대면화고위병균、면화홍부병균균사생장적억제작용최강，여친본대조균주Ma55차이불현저。
The sensitivities of Metarhizium anisopliae to carbendazim were determined by mycelia growth rate method in vitro. The results showed that among the tested strains of M. anisopliae,Ma55 was the most sensitive strain to carbendazim. The resistant levels of the carbendazim-resistant strains of M. anisopliae which induced by conidia were determined. The results showed that the resistant level of MC-2 was the highest among the carbenda-zim-resistant strains. The value of EC50 and the resistant level were 397.064 3μg/mL and 102.35,respectively. The mycelia growth rate,conidia generation and inhibition effects on Fusarium oxysporum f. sp. vasinfectum and F.moniliforme of the carbendazim-resistant strains were also studied. The results showed that the mycelium growth rate of each carbendazim-resistant strain was smaller than Ma5 5 strain(the parent strain),while the ability of conidia generation was stronger than that of Ma55. Among the carbendazim-resistant strains,the conidia gen-erated by MC-2 strain were the most,MC-5 strain followed,and MC-8 strain was the last. The inhibitory effects of the carbendazim-resistant strains against the tested F.oxysporum f. sp. vasinfectum and F.moniliforme were significant by dual-cultured,and the inhibitory effects of MC-2 was the largest.
