CAJ | 학술논문

以菌株液体发酵过程中菌丝干重和芽生孢子浓度为测试指标，对球孢白僵菌DZDC-9菌株液体发酵营养需求进行优化，结果表明，菌株生长的适宜碳源为葡萄糖，适宜氮源为酵母粉，在基础培养液中添加0．0005 mol／L KCl可以显著提高菌丝和芽生孢子浓度。通过单因素分析和正交设计对不同培养条件下菌丝生长量进行测定，结果表明，在培养温度26℃，培养液装样量1／5，初始接菌量107个／mL，转速200 r／min的培养条件下，菌株液体发酵能在60 h达到最大菌丝生长量（13．184±0．3286）g／L。利用筛选出的培养基和培养条件进行液固双相发酵试验，得到的孢子粉产量为（22．30±1．78）g／kg大米，显著高于优化之前的产量（13．04±1．90）g／kg大米。
이균주액체발효과정중균사간중화아생포자농도위측시지표，대구포백강균DZDC-9균주액체발효영양수구진행우화，결과표명，균주생장적괄의탄원위포도당，괄의담원위효모분，재기출배양액중첨가0．0005 mol／L KCl가이현저제고균사화아생포자농도。통과단인소분석화정교설계대불동배양조건하균사생장량진행측정，결과표명，재배양온도26℃，배양액장양량1／5，초시접균량107개／mL，전속200 r／min적배양조건하，균주액체발효능재60 h체도최대균사생장량（13．184±0．3286）g／L。이용사선출적배양기화배양조건진행액고쌍상발효시험，득도적포자분산량위（22．30±1．78）g／kg대미，현저고우우화지전적산량（13．04±1．90）g／kg대미。
The principal objectives of this study were to determine the optimal liquid culture conditions in shake flasks for maximal biomass and blast spores of Beauveria bassiana. The results showed that glucose was the suitable carbon source,and yeast powder was the suitable nitrogen source for the growth of strain DZDC-9. Adding 0.000 5 mol/L KCl into the submerged culture can significantly enhance the yield of biomass and blast spores. The biomass reached(13.184±0.328 6)g/L after 60 h. The best culture conditions were shake flasks with one-fifth nutrient so-lution and initial conidial concentration of 107 spores/mL in a rotary shaking incubator at 200 r/min at 26 ℃ , which could increase the biomass and shorten the incubation time. Using the selected nutrition and culture condi-tions to produce conidia,the yield could achieve 22.30±1.78 per kilogram of rice.