CAJ | 학술논문

对白头翁叶斑病菌胞壁结合黑色素和胞外黑色素进行了理化性质和红外光谱扫描测定，结果表明两者具有相似的理化性质，均易溶于KOH、H2 O2和NaClO，不溶于水、乙醇和丙酮。红外光谱分析表明，白头翁叶斑病菌YS-24菌株的胞壁结合黑色素与胞外黑色素为同一种类型的黑色素。DHN黑色素的特异性抑制剂—三环唑，对白头翁叶斑病菌黑色素的产生有明显的抑制作用；以白头翁叶斑病菌基因组DNA为模板，通过PCR扩增，得到了聚酮体合成酶基因的同源片段AaPKS，初步推断白头翁叶斑病菌黑色素合成属于DHN途径。
대백두옹협반병균포벽결합흑색소화포외흑색소진행료이화성질화홍외광보소묘측정，결과표명량자구유상사적이화성질，균역용우KOH、H2 O2화NaClO，불용우수、을순화병동。홍외광보분석표명，백두옹협반병균YS-24균주적포벽결합흑색소여포외흑색소위동일충류형적흑색소。DHN흑색소적특이성억제제—삼배서，대백두옹협반병균흑색소적산생유명현적억제작용；이백두옹협반병균기인조DNA위모판，통과PCR확증，득도료취동체합성매기인적동원편단AaPKS，초보추단백두옹협반병균흑색소합성속우DHN도경。
The characteristics of melanin,extracted from cell wall and fermented filtrate of Ascochyta anemones, were determined by diagnostic test and infrared spectroscopy. The results indicated that they have similar physical and chemical properties,and they are soluble in KOH,H2 O2 and NaClO,but not in water,ethanol and acetone. Infrared spectrum analysis showed that the wall-bound melanin and extracellular melanin belonged to the same type. Tricyclazole,a specific inhibitor of DHN melanin synthesis,could inhibit the fungal melanin’s biosynthesis of A.anemones. The homologous fragment of polyketide synthase gene (AaPKS)was amplified by PCR using A.anemones genomic DNA as a template. The results suggested that the melanin in A.anemones might be synthe-sized from DHN pathway.