临床和实验医学杂志
臨床和實驗醫學雜誌
림상화실험의학잡지
JOURNAL OF CLINICAL AND EXPERIMENTAL MEDICINE
2014年
21期
1802-1804,1805
,共4页
HBV-DNA定量%肝功损害指标%HBV-M
HBV-DNA定量%肝功損害指標%HBV-M
HBV-DNA정량%간공손해지표%HBV-M
Quantitative HBV-DNA%Liver damage index%HBV-M
目的：探讨HBV-DNA定量与HBV模式及肝功损害指标定量关系。方法随机抽选369例乙肝或疑似乙肝病毒感染者，荧光定量聚合酶链反应（ PCR）对患者血清HBV-DNA定量，酶联免疫吸附法测定乙型肝炎血清标志物模式（HBV-M），采用全自动生化仪进行天冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）、碱性磷酸酶（ALP）、γ-谷氨酰基转移酶（γ-GPT）、总胆红素（ TBIL）等肝功指标含量的检测。结果 HBV模式下HBsAg+ HBeAg+ HBcAb（大三阳）患者的血清HBV-DNA阳性检出率（93.8%）及平均含量对数值（7.31℃1.23）明显高于HBsAg+HBeAb+HBcAb（小三阳）患者（ P <0.05）；HBsAg+HBeAb+HBcAb（小三阳）患者的血清HBV-DNA阳性检出率（41.0%）及平均含量对数值（5.03℃1.31）低于HBsAg+HBcAb患者（ P <0.05），但均显著高于其他各HBV-M模式（ P <0.05）。HBV-DNA定量与AST、ALT等肝功损害指标呈正相关性（ r =0.235，P =0.014；r =0.254，P =0.009）。结论 HBV-DNA定量与HBV-M模式关系密切，与ALT、AST呈正相关性，定期检测HBV-M和HBV-DNA能全面了解HBV感染、复制以及传染性状态。
目的：探討HBV-DNA定量與HBV模式及肝功損害指標定量關繫。方法隨機抽選369例乙肝或疑似乙肝病毒感染者，熒光定量聚閤酶鏈反應（ PCR）對患者血清HBV-DNA定量，酶聯免疫吸附法測定乙型肝炎血清標誌物模式（HBV-M），採用全自動生化儀進行天鼕氨痠氨基轉移酶（AST）、丙氨痠氨基轉移酶（ALT）、堿性燐痠酶（ALP）、γ-穀氨酰基轉移酶（γ-GPT）、總膽紅素（ TBIL）等肝功指標含量的檢測。結果 HBV模式下HBsAg+ HBeAg+ HBcAb（大三暘）患者的血清HBV-DNA暘性檢齣率（93.8%）及平均含量對數值（7.31℃1.23）明顯高于HBsAg+HBeAb+HBcAb（小三暘）患者（ P <0.05）；HBsAg+HBeAb+HBcAb（小三暘）患者的血清HBV-DNA暘性檢齣率（41.0%）及平均含量對數值（5.03℃1.31）低于HBsAg+HBcAb患者（ P <0.05），但均顯著高于其他各HBV-M模式（ P <0.05）。HBV-DNA定量與AST、ALT等肝功損害指標呈正相關性（ r =0.235，P =0.014；r =0.254，P =0.009）。結論 HBV-DNA定量與HBV-M模式關繫密切，與ALT、AST呈正相關性，定期檢測HBV-M和HBV-DNA能全麵瞭解HBV感染、複製以及傳染性狀態。
목적：탐토HBV-DNA정량여HBV모식급간공손해지표정량관계。방법수궤추선369례을간혹의사을간병독감염자，형광정량취합매련반응（ PCR）대환자혈청HBV-DNA정량，매련면역흡부법측정을형간염혈청표지물모식（HBV-M），채용전자동생화의진행천동안산안기전이매（AST）、병안산안기전이매（ALT）、감성린산매（ALP）、γ-곡안선기전이매（γ-GPT）、총담홍소（ TBIL）등간공지표함량적검측。결과 HBV모식하HBsAg+ HBeAg+ HBcAb（대삼양）환자적혈청HBV-DNA양성검출솔（93.8%）급평균함량대수치（7.31℃1.23）명현고우HBsAg+HBeAb+HBcAb（소삼양）환자（ P <0.05）；HBsAg+HBeAb+HBcAb（소삼양）환자적혈청HBV-DNA양성검출솔（41.0%）급평균함량대수치（5.03℃1.31）저우HBsAg+HBcAb환자（ P <0.05），단균현저고우기타각HBV-M모식（ P <0.05）。HBV-DNA정량여AST、ALT등간공손해지표정정상관성（ r =0.235，P =0.014；r =0.254，P =0.009）。결론 HBV-DNA정량여HBV-M모식관계밀절，여ALT、AST정정상관성，정기검측HBV-M화HBV-DNA능전면료해HBV감염、복제이급전염성상태。
Objective To explore the quantitative relationship between quantification of HBV-DNA and HBV model or liver damage in-dexes. Methods A total of 369 cases of hepatitis B or suspected infection of hepatitis B virus were randomly selected for this study,fluorescence quantitative polymerase chain reaction( PCR)had been applied for quantified determination of serum HBV-DNA,enzyme linked immunosorbent assay was used to determine the serum markers of hepatitis B model( HBV-M),automatic biochemical analyzer was used to detect the levels of liver function indexes including aspartate aminotransferase( AST),alanine aminotransferase( ALT),alkaline phosphatase( ALP),gamma glu-tamyltransferase(γ -GPT)and total bilirubin( TBIL). Results Under HBV model,HBsAg+,HBeAg+ and HBcAb in serum of patients with HBV-DNA positive rate of 93. 8% and the average content(7. 31 ℃1. 23)were significantly higher than those of numerical HBsAg+, HBeAb+ and HBcAb( P <0. 05);the positive rate(40%)of HBsAg+,HBeAb+ and HBcAb in serum of patients with HBV-DNA and the average content of this value(5. 03℃1. 31)were significantly lower than those with HBsAg+ and HBcAb( P <0. 05),but it was significantly higher than that of other HBV-M model( P <0. 05). The content of HBV-DNA showed a positive correlation with ALT and AST( r =0. 235, P =0. 014;r =0. 254,P =0. 009). Conclusion The quantitative determination of HBV-DNA is closely associated with HBV-M,it is posi-tively correlated with ALT and AST,regular detection of HBV-M and HBV-DNA can comprehensively understand the status of HBV infection, replication and communicability.
