CAJ | 학술논문

目的：探究大鼠创伤性脑损伤（ TBI）后不同时间点血管生成素（ Ang）-1和Ang-2的表达情况。方法依据Marmarou方法制备脑损伤模型。在脑损伤后1 h，6 h，12 h，24 h和72 h 5个亚组中，采用改良神经功能严重程度评分系统（ mNSS）进行神经功能评分，酶联免疫吸附测定（ ELISA）法检测脑组织中Ang-1和Ang-2水平。结果 TBI伤后脑组织Ang-1含量1~24 h无变化，72 h显著上升；Ang-2含量1 h无变化，6 h开始显著上升，24 h达顶峰，72 h有下降但仍高于6 h组。重度脑损伤大鼠Ang-1和Ang-2水平较中度损伤大鼠升高早，幅度大。结论脑损伤后脑中Ang-1和Ang-2的表达呈现规律性变化，可能同继发性损伤和组织修复相关。
목적：탐구대서창상성뇌손상（ TBI）후불동시간점혈관생성소（ Ang）-1화Ang-2적표체정황。방법의거Marmarou방법제비뇌손상모형。재뇌손상후1 h，6 h，12 h，24 h화72 h 5개아조중，채용개량신경공능엄중정도평분계통（ mNSS）진행신경공능평분，매련면역흡부측정（ ELISA）법검측뇌조직중Ang-1화Ang-2수평。결과 TBI상후뇌조직Ang-1함량1~24 h무변화，72 h현저상승；Ang-2함량1 h무변화，6 h개시현저상승，24 h체정봉，72 h유하강단잉고우6 h조。중도뇌손상대서Ang-1화Ang-2수평교중도손상대서승고조，폭도대。결론뇌손상후뇌중Ang-1화Ang-2적표체정현규률성변화，가능동계발성손상화조직수복상관。
Objective To explore the expressions of angiopoietin( Ang)-1 and -2 in rats at different time points after traumatic brain injury( TBI). Methods The TBI model of rat was established by the method of Marmarou. In 1 h,6 h,12 h,24 h and 72 h 5 sub-groups after TBI,the modified neurologic severity score test( mNSS)was used to evaluate the neurological score and the levels of Ang-1 and Ang-2 in brain tissues were measured by enzyme-linked immunosorbent assay( ELISA). Results The level of Ang-1 in brain tissue after TBI did not change from 1 h to 24 h group,but increased significantly compared with 72 h group. The level of Ang-2 did not change in 1h and increased significantly from 6h,which reached the peak at 24 h. The level of Ang-2 had decreased at 72 h group which was still higher than 6 h group. The level of Ang-1 and Ang-2 in severe brain injury group increased earlier,with large magnitude,than that of medium injury group. Conclusion The expres-sions of Ang-1 and Ang-2 after TBI showed regular changes which may be related to secondary damage and tissue repair.