现代中西医结合杂志
現代中西醫結閤雜誌
현대중서의결합잡지
MODERN JOURNAL OF INTEGRATED TRADITIONAL CHINESE AND WESTERN MEDICINE
2014年
33期
3658-3661
,共4页
顾秋艳%董泽飞%范亚敏%杨洪喜%崔俊霞
顧鞦豔%董澤飛%範亞敏%楊洪喜%崔俊霞
고추염%동택비%범아민%양홍희%최준하
翼状胬肉%Rho C 蛋白%免疫组织化学
翼狀胬肉%Rho C 蛋白%免疫組織化學
익상노육%Rho C 단백%면역조직화학
pterygium%RhoC protein%immunohistochemistry
目的:研究 RhoC 蛋白在翼状胬肉中的表达情况,并以此作为标记物评估翼状胬肉的生物学特性。方法选择手术切除的45例翼状胬肉标本,应用辣根过氧化物酶标记的链霉卵白素免疫组织化学方法(S -P 法)检测 RhoC 蛋白在翼状胬肉中的表达,分析 RhoC蛋白的表达与翼状胬肉临床病理参数的关系。结果翼状胬肉组织中 RhoC 的阳性表达率为60%(27/45),显著高于正常球结膜组织(P <0.05)。31例进展期翼状胬肉 RhoC 阳性表达率为58%(18/31),14例静止期翼状胬肉阳性表达率为57%(8/14),二者比较无显著性差异(P >0.05);24例男性中14例阳性表达(58%),21例女性中13例阳性表达(62%);≥60岁患者的阳性表达率为56%(15/27),60岁以下患者的阳性表达率为61%(11/18);RhoC 蛋白在不同性别、不同年龄患者间表达均无显著性差异(P 均>0.05)。结论 RhoC 蛋白的检测可有效判断翼状胬肉发病及复发情况,为临床早期采取相关治疗措施提供参考,以减轻患者的痛苦及经济负担。
目的:研究 RhoC 蛋白在翼狀胬肉中的錶達情況,併以此作為標記物評估翼狀胬肉的生物學特性。方法選擇手術切除的45例翼狀胬肉標本,應用辣根過氧化物酶標記的鏈黴卵白素免疫組織化學方法(S -P 法)檢測 RhoC 蛋白在翼狀胬肉中的錶達,分析 RhoC蛋白的錶達與翼狀胬肉臨床病理參數的關繫。結果翼狀胬肉組織中 RhoC 的暘性錶達率為60%(27/45),顯著高于正常毬結膜組織(P <0.05)。31例進展期翼狀胬肉 RhoC 暘性錶達率為58%(18/31),14例靜止期翼狀胬肉暘性錶達率為57%(8/14),二者比較無顯著性差異(P >0.05);24例男性中14例暘性錶達(58%),21例女性中13例暘性錶達(62%);≥60歲患者的暘性錶達率為56%(15/27),60歲以下患者的暘性錶達率為61%(11/18);RhoC 蛋白在不同性彆、不同年齡患者間錶達均無顯著性差異(P 均>0.05)。結論 RhoC 蛋白的檢測可有效判斷翼狀胬肉髮病及複髮情況,為臨床早期採取相關治療措施提供參攷,以減輕患者的痛苦及經濟負擔。
목적:연구 RhoC 단백재익상노육중적표체정황,병이차작위표기물평고익상노육적생물학특성。방법선택수술절제적45례익상노육표본,응용랄근과양화물매표기적련매란백소면역조직화학방법(S -P 법)검측 RhoC 단백재익상노육중적표체,분석 RhoC단백적표체여익상노육림상병리삼수적관계。결과익상노육조직중 RhoC 적양성표체솔위60%(27/45),현저고우정상구결막조직(P <0.05)。31례진전기익상노육 RhoC 양성표체솔위58%(18/31),14례정지기익상노육양성표체솔위57%(8/14),이자비교무현저성차이(P >0.05);24례남성중14례양성표체(58%),21례녀성중13례양성표체(62%);≥60세환자적양성표체솔위56%(15/27),60세이하환자적양성표체솔위61%(11/18);RhoC 단백재불동성별、불동년령환자간표체균무현저성차이(P 균>0.05)。결론 RhoC 단백적검측가유효판단익상노육발병급복발정황,위림상조기채취상관치료조시제공삼고,이감경환자적통고급경제부담。
Objective It is to investigate the expression of RhoC protein in pterygium, and assess the biological character-istics of pterygium by using RhoC protein as a marker.Methods 45 cases of pterygium by surgical resection were choose, the expression of RhoC protein in pterygium by application of horseradish peroxidase labeled chain mildew avidin method of immu-nohistochemistry (S -P method), and the ? relationship between RhoC protein expression and clinicopathological parameters of pterygium were analyzed.Results The positive expression rate of RhoC in pterygium tissues was 60% (27/45), it was sig-nificantly higher than that in normal conjunctival tissue (Z =3.285, P <0.05).The positive expression rate RhoC in 31 ca-ses of advanced pterygium was 58% (18 /31), and was 57% (8 /14) in 14 cases of resting stage pterygium, There was signif-icant difference between them (P >0.05).14 cases of positive expression in 24 cases of male (positive rate 58%), 13 cases were positive expression in 21 cases of women (positive rate was 62%); the positive expression rate was 56% (15 /27) in pa-tients with age≥60 and was 61% (11 /18) in patients with age <60; there was no significant difference in RhoC protein ex-pression between different gender or different age of patients (all P >0.05).Conclusion RhoC protein detection can effective-ly determine the incidence and recurrence of pterygium, and provide a reference to take relevant measures in early stage for clinical, accordingly alleviate the suffering and economic burden on patients.