CAJ | 학술논문

目的：研究 RANTES 基因启动子区－403G／A、－28C／G 和内含子区 Inl．1T／C的多态性和结核病易感性之间的关系。方法采用病例对照研究方法，应用聚合酶链反应－限制性片段长度多态性（PCR －RFLP）对78例结核病患者（病例组）和97例健康对照者（对照组）的 RANTES －403G／A、－28C／G 和 Inl．1T／C 多态性进行基因分型，分析这三个位点多态性与结核病发生率的关系。结果2组 RANTES －403G／A 位点 GG、GA 和 AA 基因型的分布频率比较差异无统计学意义（χ2＝0．41，P ＞0．05），G、A 等位基因分布频率差异无统计学意义（χ2＝0．30，P ＞0．05）。2组RANTES －28C／G 位点 CC、 CG 和 GG 基因型的分布频率比较差异无统计学意义（χ2＝0．15，P ＞0．05），C、G 等位基因分布频率差异无统计学意义（χ2＝0．02，P ＞0．05）。2组 RANTES Inl．1T／C 位点 TT、TC 和 CC 基因型的分布频率比较差异无统计学意义（χ2＝0．53，P＞0．05），T、C 等位基因分布频率差异无统计学意义（χ2＝0．19，P ＞0．05）。结论 RANTES 基因启动子区的－403G／A、－28C／G 和内含子区的 Inl．1T／C 位点的单核苷酸多态性（SNP）与肺结核感染的易感性不相关。
목적：연구 RANTES 기인계동자구－403G／A、－28C／G 화내함자구 Inl．1T／C적다태성화결핵병역감성지간적관계。방법채용병례대조연구방법，응용취합매련반응－한제성편단장도다태성（PCR －RFLP）대78례결핵병환자（병례조）화97례건강대조자（대조조）적 RANTES －403G／A、－28C／G 화 Inl．1T／C 다태성진행기인분형，분석저삼개위점다태성여결핵병발생솔적관계。결과2조 RANTES －403G／A 위점 GG、GA 화 AA 기인형적분포빈솔비교차이무통계학의의（χ2＝0．41，P ＞0．05），G、A 등위기인분포빈솔차이무통계학의의（χ2＝0．30，P ＞0．05）。2조RANTES －28C／G 위점 CC、 CG 화 GG 기인형적분포빈솔비교차이무통계학의의（χ2＝0．15，P ＞0．05），C、G 등위기인분포빈솔차이무통계학의의（χ2＝0．02，P ＞0．05）。2조 RANTES Inl．1T／C 위점 TT、TC 화 CC 기인형적분포빈솔비교차이무통계학의의（χ2＝0．53，P＞0．05），T、C 등위기인분포빈솔차이무통계학의의（χ2＝0．19，P ＞0．05）。결론 RANTES 기인계동자구적－403G／A、－28C／G 화내함자구적 Inl．1T／C 위점적단핵감산다태성（SNP）여폐결핵감염적역감성불상관。
Objective It is to investigate the relationship of gene polymorphisms of RANTES promoters -403G/A, -28C/G and its intron Inl.1T/C with susceptibility to tuberculosis.Methods The gene polymorphisms of RANTES -403G/A, -28C/G and Inl.1T/C of 78 patients with tuberculosis (case group) and 97 healthy controls (control group ) were genotyped by polymerase chain reaction -restriction fragment length polymorphism (PCR -RFLP) in the case -control study.The correla-tion between gene polymorphism of them and susceptibility to tuberculosis were analyzed.Results There was no significant difference in GG, GA and AA genotype frequency of RANTES -403G/A between the case group and the control group (χ2 =0.41, P >0.05); and no significant difference in C, G allele frequency distribution between both groups (χ2 =0.02, P >0. 05).There was no significant difference in CC, CG and GG genotype frequency of RANTES -28C/G between both groups (χ2 =0.15, P >0.05); and no significant difference in C, G allele frequency distribution between both groups (χ2 =0.02, P >0.05).There was no significant difference in TT, TC and CC genotype frequency of RANTES Inl.1T/C between both groups (χ2 =0.53, P >0.05); and no significant difference in T, C allele frequency distribution between both groups (χ2 =0.19, P >0.05).Conclusion Single nucleotide polymorphism (SNP) of RANTES promoters -403G/A, -28C/G and its intron Inl.1T/C may not be related to the susceptibility to tuberculosis infection.