杭州师范大学学报(自然科学版)
杭州師範大學學報(自然科學版)
항주사범대학학보(자연과학판)
JOURNAL OF HANGZHOU NORMAL UNIVERSITY(NATURAL SCIENCE EDITION)
2014年
6期
602-605,611
,共5页
孙扬%陆文怡%张艳%王广洋%孙佳丽%薛大伟%张晓勤
孫颺%陸文怡%張豔%王廣洋%孫佳麗%薛大偉%張曉勤
손양%륙문이%장염%왕엄양%손가려%설대위%장효근
大麦%类病斑突变体%活性氧%遗传分析
大麥%類病斑突變體%活性氧%遺傳分析
대맥%류병반돌변체%활성양%유전분석
barley%lesion mimic mutant%ROS%genetic analysis
对甲基磺酸乙酯(EMS)诱变大麦品种ZJU3获得的一个类病斑突变体(bspl1)进行了遗传和生理分析。遗传分析结果显示,该表型由隐性单基因控制;苗期叶片生理生化分析表明,bspl1中SOD、POD以及CAT的活性较野生型ZJU3均明显升高。通过比较bspl1和野生型ZJU3在生理生化水平上的差异,推测类病斑的产生与叶片中活性氧的堆积相关。
對甲基磺痠乙酯(EMS)誘變大麥品種ZJU3穫得的一箇類病斑突變體(bspl1)進行瞭遺傳和生理分析。遺傳分析結果顯示,該錶型由隱性單基因控製;苗期葉片生理生化分析錶明,bspl1中SOD、POD以及CAT的活性較野生型ZJU3均明顯升高。通過比較bspl1和野生型ZJU3在生理生化水平上的差異,推測類病斑的產生與葉片中活性氧的堆積相關。
대갑기광산을지(EMS)유변대맥품충ZJU3획득적일개류병반돌변체(bspl1)진행료유전화생리분석。유전분석결과현시,해표형유은성단기인공제;묘기협편생리생화분석표명,bspl1중SOD、POD이급CAT적활성교야생형ZJU3균명현승고。통과비교bspl1화야생형ZJU3재생리생화수평상적차이,추측류병반적산생여협편중활성양적퇴적상관。
Barley lesion mimic mutant bspl1,which is derived from Ethylmethylsulfone mutagenesis of ZJU3 ,is made genetic and physiological analysis .The genetic analysis indicates that the phenotype of bspl1 is controlled by a recessive monogene .The physiological and biochemical analyses of seedling leaves show that the activity of SOD ,POD and CAT in bspl1is significantly higher than that in wild type ZJU3 .The results demonstrate that the lesion appearance of bspl1is related with the accumulation of reactive oxygen species in leaves .