CAJ | 학술논문

[目的]通过实时荧光定量 PCR分析不同环境温度和光照强度处理下，马铃薯苯丙烷类代谢途径关键酶基因，转录调节因子StR2R3-MYB和TGA基因表达情况。[方法]运用多因子及其互作逐步回归法建立光照强度、温度和处理时间影响基因表达的模式，逐步回归分析法研究 StR2R3-MYB和 TGA转录因子对苯丙烷类代谢途径关键酶基因的影响力。[结果]环境温度与 StPAL、StDFR和 StR2R3-MYB基因的表达量负相关，StTGA表达与温度正相关；环境光照强度与 StCHS、StDFR和StR2R3-MYB表达正相关。环境光照强度和温度对 StPAL和 StDFR基因表达存在显著交互作用。[结论]环境光照强度影响马铃薯苯丙烷代谢途径关键酶 StPAL和 StDFR基因对温度的响应模式。马铃薯 StR2R3-MYB转录调节因子对 StCHS 基因表达具有明显正向影响，而马铃薯 StTGA转录调节因子对 StDFR表达具有明显反向作用。
[목적]통과실시형광정량 PCR분석불동배경온도화광조강도처리하，마령서분병완류대사도경관건매기인，전록조절인자StR2R3-MYB화TGA기인표체정황。[방법]운용다인자급기호작축보회귀법건립광조강도、온도화처리시간영향기인표체적모식，축보회귀분석법연구 StR2R3-MYB화 TGA전록인자대분병완류대사도경관건매기인적영향력。[결과]배경온도여 StPAL、StDFR화 StR2R3-MYB기인적표체량부상관，StTGA표체여온도정상관；배경광조강도여 StCHS、StDFR화StR2R3-MYB표체정상관。배경광조강도화온도대 StPAL화 StDFR기인표체존재현저교호작용。[결론]배경광조강도영향마령서분병완대사도경관건매 StPAL화 StDFR기인대온도적향응모식。마령서 StR2R3-MYB전록조절인자대 StCHS 기인표체구유명현정향영향，이마령서 StTGA전록조절인자대 StDFR표체구유명현반향작용。
Objective] Expressions of key enzymatic genes involved in phenyl-propanoid metabolic pathway in potato and StR2R3-MYB and StTGA transcripters were investigated in the present study. [Method] The primitive cultivar Yan was the materials for replicated trials and total RNA extracted from tissues of seedlings. Re-al-time florescent quantification PCR, multiple intervals of air temperature, light-il umi-nation and time-duration were factors of treatments in the experiment. Data on gene expressions were obtained and proceed to asses and compare effects based on statistical analysis. [Result] The results showed negative correlations between tem-perature degrees and expressions of StPAL, StDFR and StR2R3-MYB genes but not StTGA. Positive correlations, however, were derived between those of StCHS, StDFR and StR2R3-MYB and light-intensity. Significant interactive effects between expressions of StPAL and StDFR and treatments, light intensity and temperature degree, along the phenylpropanoid pathway were observed. Transcription regulator of StR2R3-MYB showed significant positive effect on the expression of StCHS of potato. StTGA transcription factor, on the other hand, gave significant negative ef-fects on the expression of StDFR. [Conclusion] Results from present study reveal the role of environmental factors and complicate interactions between such condi-tions as temperature-light il umination and mRNA function of target genes.