农业科学与技术(英文版)
農業科學與技術(英文版)
농업과학여기술(영문판)
AGRICULTURAL SCIENCE & TECHNOLOGY
2014年
11期
1879-1882
,共4页
吴金平%丁自立%刘晓艳%郭凤领%焦忠久%邱正明
吳金平%丁自立%劉曉豔%郭鳳領%焦忠久%邱正明
오금평%정자립%류효염%곽봉령%초충구%구정명
卷丹百合%黑斑病%生物学特性%分子鉴定
捲丹百閤%黑斑病%生物學特性%分子鑒定
권단백합%흑반병%생물학특성%분자감정
Lilium lancifolium%Black spot%Biological characteristic%Molecular identifi-cation
随着卷丹百合种植面积的扩大,卷丹百合叶部真菌性病害也越来越严重,尤其在2014年6~7月份,由于降水偏多,卷丹百合叶部病害大发生,6月中旬重病田卷丹百合枯死株率达50%~70%,部分田块卷丹百合地上部全部枯死。从卷丹百合发病叶片分离得到病原菌,进行致病性鉴定、孢子形态观察、18S rDNA序列分析,生物学特性研究,结果表明,该病原菌为半知菌链格孢( Alternaria alternata)。该菌菌丝生长致死温度为55℃;适宜 pH为6~7;在供试的几种碳、氮源中,最适的碳源是麦芽糖,最适的氮源是酵母。
隨著捲丹百閤種植麵積的擴大,捲丹百閤葉部真菌性病害也越來越嚴重,尤其在2014年6~7月份,由于降水偏多,捲丹百閤葉部病害大髮生,6月中旬重病田捲丹百閤枯死株率達50%~70%,部分田塊捲丹百閤地上部全部枯死。從捲丹百閤髮病葉片分離得到病原菌,進行緻病性鑒定、孢子形態觀察、18S rDNA序列分析,生物學特性研究,結果錶明,該病原菌為半知菌鏈格孢( Alternaria alternata)。該菌菌絲生長緻死溫度為55℃;適宜 pH為6~7;在供試的幾種碳、氮源中,最適的碳源是麥芽糖,最適的氮源是酵母。
수착권단백합충식면적적확대,권단백합협부진균성병해야월래월엄중,우기재2014년6~7월빈,유우강수편다,권단백합협부병해대발생,6월중순중병전권단백합고사주솔체50%~70%,부분전괴권단백합지상부전부고사。종권단백합발병협편분리득도병원균,진행치병성감정、포자형태관찰、18S rDNA서렬분석,생물학특성연구,결과표명,해병원균위반지균련격포( Alternaria alternata)。해균균사생장치사온도위55℃;괄의 pH위6~7;재공시적궤충탄、담원중,최괄적탄원시맥아당,최괄적담원시효모。
With the increasing planting area of Lilium lancifolium, the leaf fungal dis-ease of L. lancifolium is becoming more and more serious. In June and July of 2014, the excessive rainfal leads to the serious outbreak of leaf disease of L. lanci-folium. In mid-June, the wilting rate of L. lancifolium in seriously-infected field was even up to 50%-70%. In some fields, the shoots of L. lancifolium even al wilted. The pathogen was isolated from the infected leaves of L. lancifolium. Its pathogenici-ty, spore morphology, 18S rDNA sequence and biological characteristics were stud-ied. The results showed the isolated pathogen was Alternaria alternate. The lethal temperature of mycelial growth was 55 ℃. The optimum pH was 6-7. Among the tested carbon sources and nitrogen sources, the optimum carbon source was mal-tose, and the optimum nitrogen source was yeast extract.