CAJ | 학술논문

[目的]研究紫锥菊多糖对大鼠小肠上皮细胞株 IEC-6细胞增殖的影响。[方法]以IEC-6细胞为研究对象，采用 MTT方法检测细胞增殖率，观察紫锥菊多糖对该细胞增殖的影响。[结果]紫锥菊不同剂量与不同的培养时间对 IEC-6细胞的生长均有促进作用，但是随着处理时间的延长，不同浓度的促增殖效果也不尽相同，表现为在浓度为50、200μg/ml 时增殖率随着处理时间的延长先增加后逐渐减弱，但在培养24 h后表现显著促增殖作用；浓度100μg/ml 在72 h、浓度500μg/ml 在48 h表现明显促增殖作用；经48 h处理的EPS其增殖率随浓度提高而增强。[结论]紫锥菊多糖具有促进 IEC-6细胞增殖的作用，通过对小肠上皮细胞增殖的影响而发挥对肠道黏膜吸收和免疫功能的调整作用。
[목적]연구자추국다당대대서소장상피세포주 IEC-6세포증식적영향。[방법]이IEC-6세포위연구대상，채용 MTT방법검측세포증식솔，관찰자추국다당대해세포증식적영향。[결과]자추국불동제량여불동적배양시간대 IEC-6세포적생장균유촉진작용，단시수착처리시간적연장，불동농도적촉증식효과야불진상동，표현위재농도위50、200μg/ml 시증식솔수착처리시간적연장선증가후축점감약，단재배양24 h후표현현저촉증식작용；농도100μg/ml 재72 h、농도500μg/ml 재48 h표현명현촉증식작용；경48 h처리적EPS기증식솔수농도제고이증강。[결론]자추국다당구유촉진 IEC-6세포증식적작용，통과대소장상피세포증식적영향이발휘대장도점막흡수화면역공능적조정작용。
Objective] This study was conducted to investigate the effects of Echi-nacea purpurea polysaccharides (EPS) on proliferation of rat intestinal epithelial cel IEC-6. [Method] The proliferation rate of IEC-6 cel s cultured in EPS at different concentrations and for different time was measured by MTT assay and analyzed by statistic methods. [Result] The proliferation rate of IEC-6 cel s cultured in EPS at al the concentrations and for different time was improved by different extents in com-parison with the control. In detail, 50 and 200 μg/ml EPS greatly improved the IEC-6 cel proliferation after 24 h of culture; then, the cel proliferation rate in the two treatments increased from 24 to 48 h, and declined from 48 to 72 h. The cel pro-liferation was also significantly improved by culturing in 100 μg/ml EPS for 72 h and in 500 μg/ml EPS for 48 h. After 48 h of culture, the proliferation rate of IEC-6 cel increased in a EPS dose-dependent manner. [Conclusion] EPS can promote IEC-6 cel proliferation, and thus improve the intestinal mucosal absorption and immune function of rat.