农业科学与技术(英文版)
農業科學與技術(英文版)
농업과학여기술(영문판)
AGRICULTURAL SCIENCE & TECHNOLOGY
2014年
11期
1860-1864,1887
,共6页
王小敏%何孔旺%汪伟%周忠涛%杨光远%茅爱华%俞正玉%倪艳秀
王小敏%何孔旺%汪偉%週忠濤%楊光遠%茅愛華%俞正玉%倪豔秀
왕소민%하공왕%왕위%주충도%양광원%모애화%유정옥%예염수
猪圆环病毒2型%分离%鉴定%遗传变异
豬圓環病毒2型%分離%鑒定%遺傳變異
저원배병독2형%분리%감정%유전변이
Porcine circovirus type 2%Isolation%Identification%Genetic variation
[目的]了解我国部分地区猪圆环病毒2型(PCV2)的遗传变异特性。[方法]使用细胞传代的方法对 PCR检测为猪圆环病毒2型阳性的病料进行病毒分离,并通过 PCR、IFA进行初步鉴定,并特异性扩增出该分离病毒的全基因组,并进行同源性和系统进化树分析。[结果]该研究成功分离出1株 PCV2毒株,并命名为201105ZJ株;该分离株能在 PK15细胞上增殖,特异性 PCR检测可扩增出特异性片段,经 PCV2阳性血清作用后,呈现特异性免疫荧光,其 TCID50偏低,为102.67;基因组全长1768bp,与13株参考毒株的序列同源性介于94.1%~96.8%;与 AF055392PCV2a的同源性最高,为96.8%;分离株201105ZJ 和AF055392属于基因型 PCV2a,与 PCV2c基因型亲缘关系较远。[结论]该研究中201105ZJ分离株的遗传变异特征对于疫苗的研发、PCV2致病机理的研究和华东地区 PCVAD的防控提供了理论依据。
[目的]瞭解我國部分地區豬圓環病毒2型(PCV2)的遺傳變異特性。[方法]使用細胞傳代的方法對 PCR檢測為豬圓環病毒2型暘性的病料進行病毒分離,併通過 PCR、IFA進行初步鑒定,併特異性擴增齣該分離病毒的全基因組,併進行同源性和繫統進化樹分析。[結果]該研究成功分離齣1株 PCV2毒株,併命名為201105ZJ株;該分離株能在 PK15細胞上增殖,特異性 PCR檢測可擴增齣特異性片段,經 PCV2暘性血清作用後,呈現特異性免疫熒光,其 TCID50偏低,為102.67;基因組全長1768bp,與13株參攷毒株的序列同源性介于94.1%~96.8%;與 AF055392PCV2a的同源性最高,為96.8%;分離株201105ZJ 和AF055392屬于基因型 PCV2a,與 PCV2c基因型親緣關繫較遠。[結論]該研究中201105ZJ分離株的遺傳變異特徵對于疫苗的研髮、PCV2緻病機理的研究和華東地區 PCVAD的防控提供瞭理論依據。
[목적]료해아국부분지구저원배병독2형(PCV2)적유전변이특성。[방법]사용세포전대적방법대 PCR검측위저원배병독2형양성적병료진행병독분리,병통과 PCR、IFA진행초보감정,병특이성확증출해분리병독적전기인조,병진행동원성화계통진화수분석。[결과]해연구성공분리출1주 PCV2독주,병명명위201105ZJ주;해분리주능재 PK15세포상증식,특이성 PCR검측가확증출특이성편단,경 PCV2양성혈청작용후,정현특이성면역형광,기 TCID50편저,위102.67;기인조전장1768bp,여13주삼고독주적서렬동원성개우94.1%~96.8%;여 AF055392PCV2a적동원성최고,위96.8%;분리주201105ZJ 화AF055392속우기인형 PCV2a,여 PCV2c기인형친연관계교원。[결론]해연구중201105ZJ분리주적유전변이특정대우역묘적연발、PCV2치병궤리적연구화화동지구 PCVAD적방공제공료이론의거。
Objective] This study aimed to investigate the genetic variation of porcine circovirus type 2 (PCV2) in China. [Method] The strain was isolated from infected samples by cel passage and preliminarily identified by PCR and IFA. Ful-length genome of the isolated strain was obtained by specific amplification for homology and phylogenetic analysis. [Result] A PCV2 strain was successful y isolated and named 201105ZJ, which could proliferate in PK15 cel lines. Specific fragments could be amplified by specific PCR assay. According to results of IFA assay, specif-ic immunofluorescence was observed; the TCID50 was low (102.67); the ful-length genome sequence of the isolated strain was 1 768 bp, sharing 94.1%-96.8% ho-mology with 13 reference strains; to be specific, the isolated strain exhibited the highest homology of 96.8% with AF055392PCV2a; the isolated strain 201105ZJ and reference strain AF055392 belonged to genotype PCV2a, exhibiting a distant genetic relationship with genotype PCV2c. [Conclusion] Characteristics of genetic variation of PCV2 isolate 201105ZJ provided theoretical basis for vaccine development, investi-gation of PCV2 pathogenesis, and prevention and control of porcine circovirus-as-sociated diseases (PCVAD) in East China.