CAJ | 학술논문

目的：探讨两种模式间歇低氧对大鼠血糖、胰岛素及胰腺组织Caspase-3活性及其表达的影响。方法将63只SD大鼠随机分为常氧对照组、间歇低氧1组(高频轻度)、间歇低氧2组(低频重度),3组又各自随机分为暴露1、2、4周组,每组7只。3个暴露4周组分别于0周及暴露1、2、4周检测空腹血糖；3组大鼠分别于暴露1、2、4周检测胰岛素水平,胰腺组织Caspase-3活性及其mRNA和蛋白表达水平。结果间歇低氧1组暴露4周组血糖从暴露2周开始升高,暴露4周与暴露2周比较差异无统计学意义( P>0．05)；间歇低氧2组暴露4周组血糖从暴露1周即出现升高,随着暴露时间的延长进行性增高,暴露4周较间歇低氧1组暴露4周增高( P<0．05)。间歇低氧1组血清胰岛素水平呈缓慢上升趋势,暴露4周高于常氧对照组( P<0．01)；间歇低氧2组血清胰岛素从暴露1周即开始增高,暴露2周达高峰,暴露4周下降至常氧对照组水平。间歇低氧2组胰腺组织Caspase-3活性及其mRNA和蛋白表达水平随暴露时间的延长呈进行性增高(P<0．05,P<0．01)。结论低频重度间歇低氧模式对胰腺组织的损害较高频轻度间歇低氧模式严重,且呈进行性加重。
목적：탐토량충모식간헐저양대대서혈당、이도소급이선조직Caspase-3활성급기표체적영향。방법장63지SD대서수궤분위상양대조조、간헐저양1조(고빈경도)、간헐저양2조(저빈중도),3조우각자수궤분위폭로1、2、4주조,매조7지。3개폭로4주조분별우0주급폭로1、2、4주검측공복혈당；3조대서분별우폭로1、2、4주검측이도소수평,이선조직Caspase-3활성급기mRNA화단백표체수평。결과간헐저양1조폭로4주조혈당종폭로2주개시승고,폭로4주여폭로2주비교차이무통계학의의( P>0．05)；간헐저양2조폭로4주조혈당종폭로1주즉출현승고,수착폭로시간적연장진행성증고,폭로4주교간헐저양1조폭로4주증고( P<0．05)。간헐저양1조혈청이도소수평정완만상승추세,폭로4주고우상양대조조( P<0．01)；간헐저양2조혈청이도소종폭로1주즉개시증고,폭로2주체고봉,폭로4주하강지상양대조조수평。간헐저양2조이선조직Caspase-3활성급기mRNA화단백표체수평수폭로시간적연장정진행성증고(P<0．05,P<0．01)。결론저빈중도간헐저양모식대이선조직적손해교고빈경도간헐저양모식엄중,차정진행성가중。
Objective To study the effects of two different patterns of intermittent hypoxia on glucose, insulin, and activity and expression of pancreas caspase-3 in rats. Methods Sixty-three Sprague-Dawley rats were randomly divided into control group (n=21), intermittent hypoxia group 1 (group A, high frequence and mild degree, n=21) and intermittent hypoxia group 2 (group B, low frequence and severe degree, n=21). The three groups were randomly divided into three subgroups, and were exposed for 1 week, 2 weeks and 4 weeks respectively ( n=7 for each subgroup) . Levels of fasting plasma glucose ( FPG) in the rats were measured at 0 week, and exposure for 1 week, 2 weeks and 4 weeks, and then levels of insulin in serum, caspase-3 mRNA, protein and activity of pancreas of all rats were detected after exposure for 1 week, 2 weeks and 4 weeks. Results The group A was exposured for 4 weeks, and glucose level was increased after exposure for 2 weeks, but there was no significant difference in the level between exposure for 2 weeks and 4 weeks (P>0. 05);group B was exposured for 4 weeks, and glucose level was increased after exposure for 1 week, and glucose level was progressively increased with prolonging exposure time, and the level after exposure for 4 weeks was significantly higher than that in group A (P<0. 05). Insulin level in group A showed a slowly upward tendency, and the level after exposure for 4 weeks was significantly higher than that in control group (P<0. 01); in group B, the insulin level began to rise up after exposure for 1 week, and the peak emerged after exposure for 2 weeks, and then the difference in the level showed no statistical significance after exposure for 4 weeks between group B and control group (P>0. 05). The levels of Caspase-3 activity and mRNA in group B were progressively increased with prolonging time (P<0. 05, P<0. 01). Conclusion Low frequence and severe degree of intermittent hypoxia can cause severe damage on pancreas tis- sues than that by high frequence and mild degree of intermittent hypoxia, and the damage is progressive aggravation.