CAJ | 학술논문

为明确砂仁茎枯病病原菌的分类地位，对该病原菌进行分离培养，通过致病性测定、病原菌形态特征观察及 rDNA-ITS 序列分析对病原菌进行鉴定。结果表明：砂仁茎枯病主要危害茎秆，初染病时病斑呈水渍状，逐渐扩大为纺锤形至不规则形，病斑边缘黄褐色，病健部分界明显，病斑上着生小黑点，后期病斑部质脆，呈灰褐色至灰白色干枯，导致茎部易折断，地上部分枯萎；分生孢子团奶油状，培养6～7 d 后产生菌核；分生孢子盘圆形，直径60～150μm，分生孢子梗栅栏状排列，基部褐色，向顶渐淡，具隔膜，分生孢子单个顶生，单胞，纺缍形，两端顶部钝圆，（10～18）μm×（4．5～5）μm，附着孢大量，具钝齿状裂片；分子生物学鉴定所得 rDNA 的 ITS 序列与 GenBank 中多个 Colletotrichum crassipes 菌株 ITS 序列的同源性最高，相似度为99％。引起砂仁茎枯病的病原鉴定为壳皮炭疽菌（Colletotrichum crassipes ）。
위명학사인경고병병원균적분류지위，대해병원균진행분리배양，통과치병성측정、병원균형태특정관찰급 rDNA-ITS 서렬분석대병원균진행감정。결과표명：사인경고병주요위해경간，초염병시병반정수지상，축점확대위방추형지불규칙형，병반변연황갈색，병건부분계명현，병반상착생소흑점，후기병반부질취，정회갈색지회백색간고，도치경부역절단，지상부분고위；분생포자단내유상，배양6～7 d 후산생균핵；분생포자반원형，직경60～150μm，분생포자경책란상배렬，기부갈색，향정점담，구격막，분생포자단개정생，단포，방타형，량단정부둔원，（10～18）μm×（4．5～5）μm，부착포대량，구둔치상렬편；분자생물학감정소득 rDNA 적 ITS 서렬여 GenBank 중다개 Colletotrichum crassipes 균주 ITS 서렬적동원성최고，상사도위99％。인기사인경고병적병원감정위각피탄저균（Colletotrichum crassipes ）。
To ascertain the taxonomic status of the pathogen of stem blight on A.villosum,the pathogen was isolated and its pathogenicity,morphology and rDNA-ITS sequence were analyzed in this study.The results showed that stem blight mainly damaged the stem.Water soaked lesions initially appeared at the stem,and then extended to spindle or irregular,with brown and clear edge.Lesions with conidia on the surface late got brittle,grey-brown to pale and caused stem broken and plant wilt.Conidia mission looked like cream and produced sclerotium when cultivated for 6~7 days.Acervulus was rounded with the diameter of 60~150 μm.The conidia with brown base and septate were arranged like barrier.It is a single cell,fusiform,and measured 10~18 μm× 4.5~5 μm.In molecular identification,the rDNA-ITS sequence of the pathogen showed 99% homology with several sequences of Colletotrichum crassipes . According to the morphological and molecular characteristics,the pathogen was identified as C.crassipes.