CAJ | 학술논문

为建立同时检测番茄及其土壤中甲维盐和吡虫啉残留的快速分析方法，采用丙酮/水（1∶1， V/V）提取样品，经硅胶和活性炭混合物层析柱净化，HPLC-UV 检测，对甲维盐和吡虫啉在番茄及其土壤中的残留进行了检测。结果表明：1）甲维盐和吡虫啉的混合标样添加量为0．05～1．0 mg/kg 时，甲维盐在番茄和土壤中的添加回收率分别为82．54％～86．60％和82．50％～96．21％，相对偏差分别为0．96％～3．30％和0．79％～3．93％；吡虫啉在番茄和土壤中的添加回收率分别为83．25％～94．20％和90．52％～98．39％，相对偏差分别为0．71％～1．78％和0．80％～3．24％，说明该方法的准确度和精密度均符合农药残留分析的要求，适合番茄及其土壤中甲维盐和吡虫啉的残留量检测。2）采用该方法对2013年番茄及其土壤中甲维盐、吡虫啉的残留状况进行检测表明，施药5 d 后的番茄甲维盐残留量为0．03～0．05 mg/kg，吡虫啉残留量为0．05～0．10 mg/kg；施药5 d 后，土壤中甲维盐的残留量为0．03～0．05 mg/kg，吡虫啉的残留量为0．04～0．08 mg/kg。两种农药在番茄和土壤中的残留量均未超过我国在番茄中最高残留限量标准的规定。
위건립동시검측번가급기토양중갑유염화필충람잔류적쾌속분석방법，채용병동/수（1∶1， V/V）제취양품，경규효화활성탄혼합물층석주정화，HPLC-UV 검측，대갑유염화필충람재번가급기토양중적잔류진행료검측。결과표명：1）갑유염화필충람적혼합표양첨가량위0．05～1．0 mg/kg 시，갑유염재번가화토양중적첨가회수솔분별위82．54％～86．60％화82．50％～96．21％，상대편차분별위0．96％～3．30％화0．79％～3．93％；필충람재번가화토양중적첨가회수솔분별위83．25％～94．20％화90．52％～98．39％，상대편차분별위0．71％～1．78％화0．80％～3．24％，설명해방법적준학도화정밀도균부합농약잔류분석적요구，괄합번가급기토양중갑유염화필충람적잔류량검측。2）채용해방법대2013년번가급기토양중갑유염、필충람적잔류상황진행검측표명，시약5 d 후적번가갑유염잔류량위0．03～0．05 mg/kg，필충람잔류량위0．05～0．10 mg/kg；시약5 d 후，토양중갑유염적잔류량위0．03～0．05 mg/kg，필충람적잔류량위0．04～0．08 mg/kg。량충농약재번가화토양중적잔류량균미초과아국재번가중최고잔류한량표준적규정。
The residual amount of emamectin benzoate and imidacloprid in tomato and soil were determined by HPLC-UV to establish a rapid analysis method for determining residual amount of Emamectin benzoate and Imidacloprid in tomato and soil simultaneously.The results showed that when the adding amount of the mixed sample of emamectin benzoate and imidacloprid is 0.05 ~ 1.0 mg/kg,the added recovery of emamectin benzoate in tomato and soil is 82.54% ~ 86.60% with 0.96% ~ 3.30%relative deviation and 82.50%~96.21% with 0.79%~3.93% relative deviation,and the added recovery of imidacloprid in tomato and soil is 83.25% ~ 94.20% with 0.71% ~ 1.78% relative deviation and 90.52%~98.39% with 0.80%~3.24% relative deviation respectively; which indicates that the determinining method with good accuracy and precision can meet the demand of pesticide residues analysis and be suitably used in residual determination of emamectin benzoate and imidacloprid in tomato and soil. The residual amount of emamectin benzoate and imidacloprid in tomato and soil 5d after spraying determined by the method is 0.03~0.05 mg/kgand 0.05 ~ 0.10 mg/kg,and 0.03 ~ 0.05 mg/kg and 0.04~0.08 mg/kg respectively.The residual amounts of emamectin benzoate and imidacloprid in tomato and soil do not exceed national maximum residual limit in tomato.