CAJ | 학술논문

为快速、准确地观察水稻原生质体制备及培养过程中水稻细胞壁的去除和再生情况，利用荧光增白剂 VBL 细胞壁染色液对细胞进行染色，制片后置于激发波长为345 nm、发射波长为430 nm 的荧光显微镜下观察。结果发现，在原生质体制备过程中，细胞周围发出的蓝色荧光初期表现分布均匀且荧光强烈，之后逐渐变为分布不均匀且荧光暗淡，最后蓝色荧光消失，表明纤维素逐渐被酶解去除；在原生质体培养过程中荧光情况与制备过程的刚好相反。结论：利用荧光增白剂 VBL 细胞壁染色液染色通过荧光强弱变化对比，可以快速、准确地检测水稻原生质体制备及培养过程中细胞壁的变化。
위쾌속、준학지관찰수도원생질체제비급배양과정중수도세포벽적거제화재생정황，이용형광증백제 VBL 세포벽염색액대세포진행염색，제편후치우격발파장위345 nm、발사파장위430 nm 적형광현미경하관찰。결과발현，재원생질체제비과정중，세포주위발출적람색형광초기표현분포균균차형광강렬，지후축점변위분포불균균차형광암담，최후람색형광소실，표명섬유소축점피매해거제；재원생질체배양과정중형광정황여제비과정적강호상반。결론：이용형광증백제 VBL 세포벽염색액염색통과형광강약변화대비，가이쾌속、준학지검측수도원생질체제비급배양과정중세포벽적변화。
The rice cells stained with fluorescent whitening agent VBL were observed by fluorescence microscope with 345nm excitation wavelength and 430nm emission wavelength to observe elimination and regeneration of rice cell walls during preparation and culture process of rice protoplasts rapidly and accurately.The blue fluorescence around cells represents distribution equality and intensity fluorescence at early stage,gradually mal-distribution and dim fluorescence,and finally the blue fluorescence disappears, which indicates that cellulose is gradually eliminated by enzymolysis, but the blue fluorescence performance in the protoplast culture process is just the opposite of the protoplast preparation process.In conclusion,the fluorescent whitening agent (VBL)can be used in rapid and accurate detection of rice protoplasts during the preparation and culture process.