CAJ | 학술논문

为更好地控制化风丹药母质量，建立高效液相色谱法同时测定化风丹药母中乌头碱、新乌头碱和次乌头碱含量。结果表明：同时测定化风丹中乌头碱、新乌头碱、次乌头碱的色谱条件为 Inertsil ODS-SP C18色谱柱（4．6 mm×250 mm，5μm），流动相乙腈-四氢呋喃（25∶15）A，0．1 mol/L 乙酸铵溶液（每1000 mL加冰醋酸0．5 mL）B，梯度洗脱（0～22 min，20％～23％ A，22～40 min，23％～25％ A），流速1．0 mL/min，柱温30℃，检测波长235 nm。在该条件下，乌头碱、新乌头碱、次乌头碱分别在0．258～1．548μg、0．322～1．932μg、0．280～1．680μg 呈良好的线性关系，平均回收率为98．64％（RSD 2．72％）、96．61％（RSD 1．15％）和98．80％（RSD 1．91％），该方法操作简便、准确、重复性好。
위경호지공제화풍단약모질량，건립고효액상색보법동시측정화풍단약모중오두감、신오두감화차오두감함량。결과표명：동시측정화풍단중오두감、신오두감、차오두감적색보조건위 Inertsil ODS-SP C18색보주（4．6 mm×250 mm，5μm），류동상을정-사경부남（25∶15）A，0．1 mol/L 을산안용액（매1000 mL가빙작산0．5 mL）B，제도세탈（0～22 min，20％～23％ A，22～40 min，23％～25％ A），류속1．0 mL/min，주온30℃，검측파장235 nm。재해조건하，오두감、신오두감、차오두감분별재0．258～1．548μg、0．322～1．932μg、0．280～1．680μg 정량호적선성관계，평균회수솔위98．64％（RSD 2．72％）、96．61％（RSD 1．15％）화98．80％（RSD 1．91％），해방법조작간편、준학、중복성호。
The content of aconitine,mesaconitine and hypaconitine in Huafeng pill was detected by HPLC to further control quality of Huafeng pill.The results showed that the chromatographic condition of detecting content of aconitine,mesaconitine and hypaconitine in Huafeng pill includes Inertsil ODS-SP C18 chromatographic column (4.6 mm× 250 mm,5 μm),gradient elution by mobile phase A:acetonitrile/tetrahydrofuran (25 ∶ 15)and mobile phase B:0.1 mol/L ammonium acetate (containing 0.5mL glacial acetic acid per 1000 mL),1.0 mL/min flow velocity,30℃ column temperature and 235nm determination wavelength.There is a good linear relation under the above-mentioned chromatographic condition when the content of aconitine,mesaconitine and hypaconitine is 0.258~ 1.548 μg,0.322~ 1.932 μg and 0.280~1.680 μg respectively.The average recovery rate of aconitine,mesaconitine and hypaconitine is 98.64 %(RSD 2.72 %),96.61 %(RSD 1.15 %)and 98.80 %(RSD 1.91 %)separately,which indicates that the detection method has the advantages of easy operation,accuracy and good repeatability.