CAJ | 학술논문

为海南安诺兰植物种质资源研究提供技术支持，以海南特有安诺兰种质为试验材料，采用正交试验，对影响 RAPD 分子标记的模板 DNA、dNTPs、Taq 聚合酶、Mg2＋浓度和引物浓度5个因素进行优化，建立适合于安诺兰 RAPD 标记的反应体系。结果表明：5个因素优化后在20μL 反应体积的浓度分别为模板 DNA 60 ng，dNTPs 150μmol/L，Taq 酶1．0 U，Mg2＋2．0 mmol/L，引物0．5μmol/L。
위해남안낙란식물충질자원연구제공기술지지，이해남특유안낙란충질위시험재료，채용정교시험，대영향 RAPD 분자표기적모판 DNA、dNTPs、Taq 취합매、Mg2＋농도화인물농도5개인소진행우화，건립괄합우안낙란 RAPD 표기적반응체계。결과표명：5개인소우화후재20μL 반응체적적농도분별위모판 DNA 60 ng，dNTPs 150μmol/L，Taq 매1．0 U，Mg2＋2．0 mmol/L，인물0．5μmol/L。
Five factors to influence the concentration of DNA template,dNTPs,Taq polymerase, Mg2+ and primer of Anota hainanensis RAPD molecular marker were optimized by the orthogonal design to build the suitable reaction system of Anota hainanensis RAPD marker and to provide the technological support for research of Anota hainanensis germplasm resources.The results showed that the optimum concentration of DNA template,dNTPs,Taq polymerase,Mg2+ and primer is 60 ng,150 μmol/L,1.0 U, 2.0 mmol/L and 0.5 μmol/L separately.