贵州农业科学
貴州農業科學
귀주농업과학
GUIZHOU AGRICULTURAL SCIENCES
2014年
11期
29-32
,共4页
彭莉萍%牛宪立%姬可平%王燕
彭莉萍%牛憲立%姬可平%王燕
팽리평%우헌립%희가평%왕연
红树%PCR%ITS 序列
紅樹%PCR%ITS 序列
홍수%PCR%ITS 서렬
mangrove%PCR%ITS sequence
为红树林植物引进优良种质资源，用改良 CTAB 法分别提取生长于广东珠海淇澳岛红树林湿地公园的3种红树基因组 DNA，以真核生物 rDNA ITS 序列的通用引物分别对其 ITS 区进行 PCR 扩增后测序，对测序结果进行比对、分析。结果表明，不同来源红树的 rDNA ITS 序列存在扩增碱基差异，无瓣海桑、尖叶卤蕨和猫尾木的 rDNA ITS 序列长度分别为731 bp、615 bp 和707 bp，将3种红树植物 ITS 序列测定结果提交到 GenBank，获得了登录号分别为 KJ161168，KJ161166，KJ161167。
為紅樹林植物引進優良種質資源，用改良 CTAB 法分彆提取生長于廣東珠海淇澳島紅樹林濕地公園的3種紅樹基因組 DNA，以真覈生物 rDNA ITS 序列的通用引物分彆對其 ITS 區進行 PCR 擴增後測序，對測序結果進行比對、分析。結果錶明，不同來源紅樹的 rDNA ITS 序列存在擴增堿基差異，無瓣海桑、尖葉滷蕨和貓尾木的 rDNA ITS 序列長度分彆為731 bp、615 bp 和707 bp，將3種紅樹植物 ITS 序列測定結果提交到 GenBank，穫得瞭登錄號分彆為 KJ161168，KJ161166，KJ161167。
위홍수림식물인진우량충질자원，용개량 CTAB 법분별제취생장우엄동주해기오도홍수림습지공완적3충홍수기인조 DNA，이진핵생물 rDNA ITS 서렬적통용인물분별대기 ITS 구진행 PCR 확증후측서，대측서결과진행비대、분석。결과표명，불동래원홍수적 rDNA ITS 서렬존재확증감기차이，무판해상、첨협서궐화묘미목적 rDNA ITS 서렬장도분별위731 bp、615 bp 화707 bp，장3충홍수식물 ITS 서렬측정결과제교도 GenBank，획득료등록호분별위 KJ161168，KJ161166，KJ161167。
The genome DNA of three mangrove species from Qi’ao island ,Guangdong was extracted by the modified CTAB method,then the rDNA ITS amplified by PCR with eukaryotic Ponsensus primers was sequenced,and finally the sequencing results were compared and analyzed to provide a reference for introduction of excellent mangrove germplasm resources.There is difference in the amplified base of rDNA ITS sequence among different mangrove species.The rDNA ITS sequence length of Sonneratia apetala , A.speciosum and D.caudafelina is 731 bp,615 bp and 707 bp respectively.The accession number of ITS sequence of S.apetala,A.speciosum and D.caudafelina is KJ161168,KJ161166 and KJ161167 in GenBank separately.
