CAJ | 학술논문

为研究黄皮果实中查尔酮合成酶（CHS）的功能，以黄皮果实的 cDNA 和 DNA 为模板，采用同源克隆的方法克隆得 CHS 基因后进行序列比对和聚类分析。结果表明：该基因的开放阅读框为1032 bp，编码343个氨基酸。基因组扩增得到了1100 bp 的片段，不含内含子。该基因主要在果实中表达，而叶片中表达较其他组织低，初步推断该基因可能与果实和花朵中的黄酮类物质合成有密切的关系。该基因编码的蛋白与柑桔的 CHS 蛋白序列亲缘关系较近，可与柑桔、葡萄、芍药、牡丹、荔枝、龙眼等聚为一类。
위연구황피과실중사이동합성매（CHS）적공능，이황피과실적 cDNA 화 DNA 위모판，채용동원극륭적방법극륭득 CHS 기인후진행서렬비대화취류분석。결과표명：해기인적개방열독광위1032 bp，편마343개안기산。기인조확증득도료1100 bp 적편단，불함내함자。해기인주요재과실중표체，이협편중표체교기타조직저，초보추단해기인가능여과실화화타중적황동류물질합성유밀절적관계。해기인편마적단백여감길적 CHS 단백서렬친연관계교근，가여감길、포도、작약、모단、려지、용안등취위일류。
In order to study the function of CHS gene,a CHS gene was cloned from wampee fruit by homology cloning method taking cDNA and DNA of wampee fruit as the template, then sequence comparison and clustering analysis was conducted.Results:The open reading frame was 1032 bp,and encoding 343 amino acids.The 1100bp fragment was amplified from the genome,the gene did not contain introns.The expression of CHS gene was mainly in fruit,and in flowers was higher than in leaves.It was inferred that the gene may be involved in the flavonoids synthesis of fruit and flowers.The cluster analysis showed that the gene encoding protein had near relationship with citrus,considered as a group with orange,grape,peony,litchi,longan,and other groups.