化工技术与开发
化工技術與開髮
화공기술여개발
TECHNOLOGY & DVELOPMENT OF CHEMICAL INDUSTRY
2014年
11期
4-7
,共4页
菠萝皮%抑菌%提取条件
菠蘿皮%抑菌%提取條件
파라피%억균%제취조건
pineapple peel%antimicrobial activity%technology conditions
采用正交试验法研究丙酮提取菠萝皮抑菌物质的工艺条件。实验结果表明，提取物对大肠杆菌、黑曲霉、金黄色葡萄球菌具有明显的抑制作用，对桔青霉则无抑制作用。对大肠杆菌和黑曲霉的抑菌效果最佳的提取工艺条件为：料液比1∶30，丙酮浓度75%，提取时间40 min，提取温度70℃。对金黄色葡萄球菌最佳的提取工艺条件为：料液比1∶10，丙酮浓度65%，提取时间40 min，提取温度50℃。在优化条件下，菠萝皮的丙酮提取物对大肠杆菌、黑曲霉、金黄色葡萄球菌的MIC分别为60、100、250mg·mL-1。
採用正交試驗法研究丙酮提取菠蘿皮抑菌物質的工藝條件。實驗結果錶明，提取物對大腸桿菌、黑麯黴、金黃色葡萄毬菌具有明顯的抑製作用，對桔青黴則無抑製作用。對大腸桿菌和黑麯黴的抑菌效果最佳的提取工藝條件為：料液比1∶30，丙酮濃度75%，提取時間40 min，提取溫度70℃。對金黃色葡萄毬菌最佳的提取工藝條件為：料液比1∶10，丙酮濃度65%，提取時間40 min，提取溫度50℃。在優化條件下，菠蘿皮的丙酮提取物對大腸桿菌、黑麯黴、金黃色葡萄毬菌的MIC分彆為60、100、250mg·mL-1。
채용정교시험법연구병동제취파라피억균물질적공예조건。실험결과표명，제취물대대장간균、흑곡매、금황색포도구균구유명현적억제작용，대길청매칙무억제작용。대대장간균화흑곡매적억균효과최가적제취공예조건위：료액비1∶30，병동농도75%，제취시간40 min，제취온도70℃。대금황색포도구균최가적제취공예조건위：료액비1∶10，병동농도65%，제취시간40 min，제취온도50℃。재우화조건하，파라피적병동제취물대대장간균、흑곡매、금황색포도구균적MIC분별위60、100、250mg·mL-1。
The technology conditions of antimicrobial substance extracted by acetone from pineapple peel were studied by orthogo-nal test. The results showed that extractive had strong antibacterial activities towards Escherichia coli, Aspergillus niger and Staphy-lococcus aureus, while no inhibitory effect on Penicillium citrinum. Toward to Escherichia coli, Aspergillus niger, the best restrain-ing technological conditions were:ratio of materialsand solvent 1∶30, acetone concentration 75%, extraction time 40min, extraction temperature 70℃. Toward to Staphylococcus aureus, the ratio of materialsand solvent was 1∶10,acetone concentration was 65%, extraction time was 40 min,extraction temperature was 50℃. Under the optimized conditions, toward to Escherichia coli, Asper-gillus niger and Staphylococcus aureusthe, the minimum inhibitory concentration of acetone extraction from pineapple peel were 40, 100, 250mg/mL respectively.
