中国全科医学
中國全科醫學
중국전과의학
CHINESE GENERAL PRACTICE
2014年
12期
1407-1409
,共3页
杨晶%吕品田%李祝兵%邝相如
楊晶%呂品田%李祝兵%鄺相如
양정%려품전%리축병%광상여
肺疾病,慢性阻塞性%白介素 18%T 淋巴细胞,调节性
肺疾病,慢性阻塞性%白介素 18%T 淋巴細胞,調節性
폐질병,만성조새성%백개소 18%T 림파세포,조절성
Pulmonary disease,chronic obstructive%Interleukin - 18%T - lymphocytes,regulatory
目的：探讨吸烟慢性阻塞性肺疾病（COPD）稳定期患者、健康吸烟者及健康非吸烟者外周血中白介素（IL）-18及 Treg 细胞变化及其临床意义。方法选择2012年11月—2013年5月于河北省人民医院呼吸内科门诊就诊的吸烟 COPD 患者33例为吸烟 COPD 组，体检中心体检健康者分为健康吸烟组19例和健康非吸烟组16例。采用酶联免疫吸附测定（ELISA）法检测受试者外周血中IL -18、IL -17水平，用流式细胞术检测 CD8+及 Treg 细胞比例。结果吸烟 COPD 组IL -18、IL -17、CD8+较健康吸烟组、健康非吸烟组均升高，Treg 细胞较健康吸烟组下降、较健康非吸烟组升高（P <0,05）；健康吸烟组IL -18、IL -17、CD8+及 Treg 细胞较健康非吸烟组均升高（ P <0,05）。结论 IL -18分泌增多，诱导IL -17、CD8+细胞上升，超出 Treg 细胞的抑制能力以及 Treg 细胞的反应钝化可能导致吸烟COPD 患者气道的慢性炎症。
目的：探討吸煙慢性阻塞性肺疾病（COPD）穩定期患者、健康吸煙者及健康非吸煙者外週血中白介素（IL）-18及 Treg 細胞變化及其臨床意義。方法選擇2012年11月—2013年5月于河北省人民醫院呼吸內科門診就診的吸煙 COPD 患者33例為吸煙 COPD 組，體檢中心體檢健康者分為健康吸煙組19例和健康非吸煙組16例。採用酶聯免疫吸附測定（ELISA）法檢測受試者外週血中IL -18、IL -17水平，用流式細胞術檢測 CD8+及 Treg 細胞比例。結果吸煙 COPD 組IL -18、IL -17、CD8+較健康吸煙組、健康非吸煙組均升高，Treg 細胞較健康吸煙組下降、較健康非吸煙組升高（P <0,05）；健康吸煙組IL -18、IL -17、CD8+及 Treg 細胞較健康非吸煙組均升高（ P <0,05）。結論 IL -18分泌增多，誘導IL -17、CD8+細胞上升，超齣 Treg 細胞的抑製能力以及 Treg 細胞的反應鈍化可能導緻吸煙COPD 患者氣道的慢性炎癥。
목적：탐토흡연만성조새성폐질병（COPD）은정기환자、건강흡연자급건강비흡연자외주혈중백개소（IL）-18급 Treg 세포변화급기림상의의。방법선택2012년11월—2013년5월우하북성인민의원호흡내과문진취진적흡연 COPD 환자33례위흡연 COPD 조，체검중심체검건강자분위건강흡연조19례화건강비흡연조16례。채용매련면역흡부측정（ELISA）법검측수시자외주혈중IL -18、IL -17수평，용류식세포술검측 CD8+급 Treg 세포비례。결과흡연 COPD 조IL -18、IL -17、CD8+교건강흡연조、건강비흡연조균승고，Treg 세포교건강흡연조하강、교건강비흡연조승고（P <0,05）；건강흡연조IL -18、IL -17、CD8+급 Treg 세포교건강비흡연조균승고（ P <0,05）。결론 IL -18분비증다，유도IL -17、CD8+세포상승，초출 Treg 세포적억제능력이급 Treg 세포적반응둔화가능도치흡연COPD 환자기도적만성염증。
Objective To explore changes and clinical significances of IL - 18 and Treg in peripheral blood of patients with stable chronic obstructive pulmonary disease(COPD),healthy smokers and healthy control non - smokers, Methods 33 smoker patients with COPD(COPD group),19 healthy smokers(healthy smoker group)and 16 healthy non - smokers(healthy non - smoker group)admitted to the Department of Respiratory Medicine of Hebei General Hospital from November 2012 to May 2013 were selected, ELISA method was used to determine IL - 18 and IL - 17 in peripheral blood,and flow cytometry method was used to determine CD8 + and Treg, Results The IL - 18,IL - 17 and CD8 + in COPD group was higher than the healthy smoker group and healthy non - smoker group,and Treg in COPD group was lower than the healthy smoker group,but higher than the healthy non - smoker group(P < 0, 05), The IL - 18,IL - 17,CD8 + and Treg were higher in healthy smoker group than the healthy non - smoker group(P < 0, 05), Conclusion Patients with COPD have an increase in IL - 18,IL - 17,CD8 + cells with a blunted Treg cells response,resulting in chronic airway inflammation.
