CAJ | 학술논문

目的：探讨 p65小干扰 RNA（siRNA）对人脐静脉内皮细胞再灌注损伤后趋化因子 CXCL16表达的调节机制。方法分4组对人脐静脉内皮细胞进行培养和处理，即对照组（正常培养基培养）、缺血再灌注组（以模拟缺血培养液培养30 min 后换正常培养液再培养4 h）、p65 siRNA 转染组（正常内皮细胞培养基培养+ p65 siRNA 转染）、缺血再灌注+ p65 siRNA 转染组（模拟缺血再灌注+ p65 siRNA 转染）。观察4组核因子κB p65、CXCL16 mRNA 相对表达量及 CXCL16表达水平以及细胞存活率。结果对照组、p65 siRNA 转染组及缺血再灌注+ p65 siRNA 转染组核因子κB p65、CXCL16 mRNA 相对表达量及 CXCL16表达水平较缺血再灌注组均降低（ P <0,05）。WST -1法测定，p65 siRNA 转染组及缺血再灌注+ p65 siRNA 转染组细胞存活率较缺血再灌注组均增高（P <0,05）。结论 p65 siRNA 通过沉默 p65，抑制模拟缺血再灌注诱导的 CXCL16表达，有利于细胞生存。
목적：탐토 p65소간우 RNA（siRNA）대인제정맥내피세포재관주손상후추화인자 CXCL16표체적조절궤제。방법분4조대인제정맥내피세포진행배양화처리，즉대조조（정상배양기배양）、결혈재관주조（이모의결혈배양액배양30 min 후환정상배양액재배양4 h）、p65 siRNA 전염조（정상내피세포배양기배양+ p65 siRNA 전염）、결혈재관주+ p65 siRNA 전염조（모의결혈재관주+ p65 siRNA 전염）。관찰4조핵인자κB p65、CXCL16 mRNA 상대표체량급 CXCL16표체수평이급세포존활솔。결과대조조、p65 siRNA 전염조급결혈재관주+ p65 siRNA 전염조핵인자κB p65、CXCL16 mRNA 상대표체량급 CXCL16표체수평교결혈재관주조균강저（ P <0,05）。WST -1법측정，p65 siRNA 전염조급결혈재관주+ p65 siRNA 전염조세포존활솔교결혈재관주조균증고（P <0,05）。결론 p65 siRNA 통과침묵 p65，억제모의결혈재관주유도적 CXCL16표체，유리우세포생존。
Objective To investigate the regulation mechanism of CXCL16 under the condition of mimic ischemia reperfusion in human umbilical vein endothelial cells(HUVEC)by utilizing p65 siRNA, Methods HUVEC were divided into 4 groups:control group(HUVEC were cultured with normal medium),mimic ischemia reperfusion group(HUVEC were cultured with mimic ischemic medium for 30 min,then were cultured with normal medium for 4 h),p65 siRNA group(HUVEC were cultured with normal medium,and were transfected with p65 siRNA),mimic ischemia reperfusion + p65 siRNA group (HUVEC were transfected with p65 siRNA,48 h later,HUVEC were cultured with mimic ischemia medium), The relative ex-pression quantity of κB p65 and CXCL16 mRNA,the expression level of CXCL16 and cell survival rate were investigated, Results The expression quantity of κB p65 and CXCL16 and the expression level of CXCL16 in control group,p65 siRNA group and mimic ischemia reperfusion + p65 siRNA group were significantly lower than those in mimic ischemia reperfusion group( P <0, 05), Meanwhile,by the method of WST - 1 assay,cell survival rate in p65 siRNA group and mimic ischemia reperfusion +p65 siRNA group were significantly higher than that in mimic ischemia reperfusion group(P < 0, 05), Conclusion By silencing p65,p65 siRNA can inhibit mimic ischemic reperfusion - induced expression level of CXCL16,which brings benefits to the survival of HUVEC.