CAJ | 학술논문

目的：对比观察电针“四关”组穴及其相应单穴对卒中后抑郁( post-stroke depression, PSD)模型大鼠行为学能力及脑单胺神经递质含量的影响,探讨“四关”组穴的协同效应及可能的作用机制。方法雄性SD大鼠50只,其中空白组10只,造模成功的32只随机分为模型组、四关组、合谷组、太冲组,每组8只。采用大脑中动脉闭阻术后结合慢性不可预见性温和应激刺激及孤养法造PSD模型。治疗组大鼠分别使用电针“四关”、合谷、太冲穴治疗,30分钟/次/日,连续21天。采用糖水试验、敞箱试验观察大鼠行为学指标变化,采用高效液相色谱仪荧光检测法观察大鼠脑组织中下丘脑区去甲肾上腺素( noradrenaline, NE )、多巴胺( dopamine, DA )、5-羟色胺(5-hydroxytryptamine,5-HT)含量。结果(1)造模后,SD大鼠行为学指标及脑单胺神经递质含量较空白组均明显减少(P<0．01)；(2)治疗后,四关组及太冲组大鼠的敞箱试验数据高于模型组(P<0．01),且太冲组直立次数治疗后与空白组无差别,而合谷组基本无变化且治疗后与模型组无差异(P>0．05)；治疗三组糖水消耗比逐步上升,且合谷组治疗后达到了与空白组无差别的水平(P>0．05)；(3)治疗后,四关组及太冲组大鼠脑单胺神经递质含量均显著增高(P<0．01),太冲组的NE、5-HT含量又明显高于四关组(P<0．05),而合谷组大鼠脑单胺神经递质含量显著低于四关组及太冲组(P<0．01)。结论电针四关组穴及太冲穴均能显著增加PSD模型大鼠的行为学指标,其作用机制可能与下丘脑组织中单胺类神经递质的含量的增加有关；但四关穴治疗该病的协同效应并不突出,其机理有待进一步研究。
목적：대비관찰전침“사관”조혈급기상응단혈대졸중후억욱( post-stroke depression, PSD)모형대서행위학능력급뇌단알신경체질함량적영향,탐토“사관”조혈적협동효응급가능적작용궤제。방법웅성SD대서50지,기중공백조10지,조모성공적32지수궤분위모형조、사관조、합곡조、태충조,매조8지。채용대뇌중동맥폐조술후결합만성불가예견성온화응격자격급고양법조PSD모형。치료조대서분별사용전침“사관”、합곡、태충혈치료,30분종/차/일,련속21천。채용당수시험、창상시험관찰대서행위학지표변화,채용고효액상색보의형광검측법관찰대서뇌조직중하구뇌구거갑신상선소( noradrenaline, NE )、다파알( dopamine, DA )、5-간색알(5-hydroxytryptamine,5-HT)함량。결과(1)조모후,SD대서행위학지표급뇌단알신경체질함량교공백조균명현감소(P<0．01)；(2)치료후,사관조급태충조대서적창상시험수거고우모형조(P<0．01),차태충조직립차수치료후여공백조무차별,이합곡조기본무변화차치료후여모형조무차이(P>0．05)；치료삼조당수소모비축보상승,차합곡조치료후체도료여공백조무차별적수평(P>0．05)；(3)치료후,사관조급태충조대서뇌단알신경체질함량균현저증고(P<0．01),태충조적NE、5-HT함량우명현고우사관조(P<0．05),이합곡조대서뇌단알신경체질함량현저저우사관조급태충조(P<0．01)。결론전침사관조혈급태충혈균능현저증가PSD모형대서적행위학지표,기작용궤제가능여하구뇌조직중단알류신경체질적함량적증가유관；단사관혈치료해병적협동효응병불돌출,기궤리유대진일보연구。
Objective To observe the effect of electroacupuncture( EA) of “Si-guan” points[ bi-lateral “Hegu”(LI 4)-“Taichong”(LR 3)] and corresponding single point on ethology and cerebral mono-amine neurotransmitters in Post-depression( PSD) Rats;to investigate the synergistic effect and the possible mechanism of bilateral LI 4-LR 3. Methods Control group had 10 normal SD rats. 32 model SD rats were quadratic randomly divided into five groups (8 rats/group):model, EA group-1 (“Si-guan” points), EA group-2 ( bilateral LI 4 points) , EA group-3 ( bilateral LR 3 points) . The PSD model was established by separately breeding and chronic unpredictable moderate stress ( CUMS) after middle cerebral artery occlu-sion (MACO) . EA(30 min) LI 4-LR 3, bilateral LI 4 and bilateral LR 3 were applied in group-1, EA group-2 and EA group-3 respectively, once daily, continuously for 21 days. The indexes of the behaviors of PSD model rats were measured by the methods of Open-Field test and Sucrose solution consumption test, the fluorometer detecting system of HPLC was used to mensurate the monoamine neurotransmitters of Rat Hypothalamus. Results Compared with control group, the data of ethology and monoamine neurotransmit-ters all decreased obviously after making model(P<0. 01). Compared with the model group ,the data of Open-Field test increased in EA group-1 and EA group-3(P<0. 01),and there was no significant differ-ence on rearing movement times in control group and EA group-3, but EA group-2 unchanged generally and indistinctive with model group(P>0. 05). The data of Sucrose solution consumption test in EA group were all increased significantly during treatment, and there was no difference in EA group-2 and control group af-ter treatment(P>0. 05. The monoamine in EA group-1 and EA group-3 were significantly increased(P<0. 01), the NE、5-HT content of EA group-3 were significantly higher than EA group-1(P<0. 05), but the monoamine neurotransmitters in EA group-2 were significantly lower than EA group-1 and EA group-3(P<0. 01). Conclusion Both of EA “Si-guan” points and bilateral “Taichong” points can significantly in-crease the Open-Field test data of PSD rats, the mechanism neurotransmitters perhaps related to the in-crease of monoamine in Rat Hypothalamus; But the synergistic effect in treatment of bilateral “Si-guan”points was not prominent, the mechanism is worth further studying.