CAJ | 학술논문

目的：研究藏茴香水提物对大鼠血脂调节及肝脏的保护作用。方法将10 w 龄雄性 SD 大鼠48只，除空白对照组8只大鼠以外其余各组大鼠均采用高脂饲料喂养8 w 后，断尾采血测血脂，确定造模成功后随机分为模型对照组、血脂康对照组、空白对照组及藏茴香水提物高、中、低剂量组（300、150、75 mg·kg-1·d-1），连续灌胃给药6 w，1次/d，模型对照组与空白对照组灌胃给予等量的生理盐水。每2周测1次体质量；末次给药后腹主动脉采血，测血清中的高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）、总胆固醇（TC）、甘油三酯（TG）等血脂水平，测定谷草转氨酶（AST）、谷丙转氨酶（ALT）等脂类酶的活性及谷胱甘肽过氧化酶（GSH-Px）、超氧化物歧化酶（SOD）、脂肪过氧化物丙二醛（MDA）等抗氧化能力的变化。结果与空白对照组比较，模型对照组 TG、TC、LDL-C 均有显著升高，HDL-C 显著降低，差异均有统计学意义（P ＜0．05）。藏茴香各剂量组 TC、TG、LDL-C 逐渐降低，HDL-C 逐渐升高（说明藏茴香干预能有效调节血脂水平）。与模型对照组比较，藏茴香各剂量组大鼠血清血脂水平差异均有统计学意义。与空白对照组比较，模型对照组血清 AST、ALT 明显升高，有统计学差异（P ＜0．05），血脂康对照组及藏茴香水提物各剂量组血清 AST、ALT 均显著降低；与模型对照组比较，藏茴香水提物高、中剂量组有统计学差异（P ＜0．05～0．01），与小剂量组比较无统计学差异（P ＞0．05）。与空白对照组比较，模型对照组 SOD、MDA 水平升高，差异有统计学意义（P ＜0．05）；与模型对照组比较，藏茴香水提物高剂量组SOD、MDA 水平和血脂康对照组 SOD 水平差异有统计学意义（P ＜0．05），与藏茴香水取物中、低剂量组比较无统计学差异（P ＞0．05）。模型对照组与空白对照组比较 GSH-Px 有统计学差异（P ＜0．001）；随着实验的进行，藏茴香水提物各剂量组 GSH-Px 均明显升高，模型对照组与血脂康对照组及藏茴香水取物高剂量组比较有统计学差异（P ＜0．05），与中剂量及低剂量组比较无统计学差异（P ＞0．05）。结论高、中剂量藏茴香水提物可明显调节机体脂质代谢，对预防和治疗高脂血症有一定的应用价值。目的：研究藏茴香水提物對大鼠血脂調節及肝髒的保護作用。方法將10 w 齡雄性 SD 大鼠48隻，除空白對照組8隻大鼠以外其餘各組大鼠均採用高脂飼料餵養8 w 後，斷尾採血測血脂，確定造模成功後隨機分為模型對照組、血脂康對照組、空白對照組及藏茴香水提物高、中、低劑量組（300、150、75 mg·kg-1·d-1），連續灌胃給藥6 w，1次/d，模型對照組與空白對照組灌胃給予等量的生理鹽水。每2週測1次體質量；末次給藥後腹主動脈採血，測血清中的高密度脂蛋白膽固醇（HDL-C）、低密度脂蛋白膽固醇（LDL-C）、總膽固醇（TC）、甘油三酯（TG）等血脂水平，測定穀草轉氨酶（AST）、穀丙轉氨酶（ALT）等脂類酶的活性及穀胱甘肽過氧化酶（GSH-Px）、超氧化物歧化酶（SOD）、脂肪過氧化物丙二醛（MDA）等抗氧化能力的變化。結果與空白對照組比較，模型對照組 TG、TC、LDL-C 均有顯著升高，HDL-C 顯著降低，差異均有統計學意義（P ＜0．05）。藏茴香各劑量組 TC、TG、LDL-C 逐漸降低，HDL-C 逐漸升高（說明藏茴香榦預能有效調節血脂水平）。與模型對照組比較，藏茴香各劑量組大鼠血清血脂水平差異均有統計學意義。與空白對照組比較，模型對照組血清 AST、ALT 明顯升高，有統計學差異（P ＜0．05），血脂康對照組及藏茴香水提物各劑量組血清 AST、ALT 均顯著降低；與模型對照組比較，藏茴香水提物高、中劑量組有統計學差異（P ＜0．05～0．01），與小劑量組比較無統計學差異（P ＞0．05）。與空白對照組比較，模型對照組 SOD、MDA 水平升高，差異有統計學意義（P ＜0．05）；與模型對照組比較，藏茴香水提物高劑量組SOD、MDA 水平和血脂康對照組 SOD 水平差異有統計學意義（P ＜0．05），與藏茴香水取物中、低劑量組比較無統計學差異（P ＞0．05）。模型對照組與空白對照組比較 GSH-Px 有統計學差異（P ＜0．001）；隨著實驗的進行，藏茴香水提物各劑量組 GSH-Px 均明顯升高，模型對照組與血脂康對照組及藏茴香水取物高劑量組比較有統計學差異（P ＜0．05），與中劑量及低劑量組比較無統計學差異（P ＞0．05）。結論高、中劑量藏茴香水提物可明顯調節機體脂質代謝，對預防和治療高脂血癥有一定的應用價值。
목적：연구장회향수제물대대서혈지조절급간장적보호작용。방법장10 w 령웅성 SD 대서48지，제공백대조조8지대서이외기여각조대서균채용고지사료위양8 w 후，단미채혈측혈지，학정조모성공후수궤분위모형대조조、혈지강대조조、공백대조조급장회향수제물고、중、저제량조（300、150、75 mg·kg-1·d-1），련속관위급약6 w，1차/d，모형대조조여공백대조조관위급여등량적생리염수。매2주측1차체질량；말차급약후복주동맥채혈，측혈청중적고밀도지단백담고순（HDL-C）、저밀도지단백담고순（LDL-C）、총담고순（TC）、감유삼지（TG）등혈지수평，측정곡초전안매（AST）、곡병전안매（ALT）등지류매적활성급곡광감태과양화매（GSH-Px）、초양화물기화매（SOD）、지방과양화물병이철（MDA）등항양화능력적변화。결과여공백대조조비교，모형대조조 TG、TC、LDL-C 균유현저승고，HDL-C 현저강저，차이균유통계학의의（P ＜0．05）。장회향각제량조 TC、TG、LDL-C 축점강저，HDL-C 축점승고（설명장회향간예능유효조절혈지수평）。여모형대조조비교，장회향각제량조대서혈청혈지수평차이균유통계학의의。여공백대조조비교，모형대조조혈청 AST、ALT 명현승고，유통계학차이（P ＜0．05），혈지강대조조급장회향수제물각제량조혈청 AST、ALT 균현저강저；여모형대조조비교，장회향수제물고、중제량조유통계학차이（P ＜0．05～0．01），여소제량조비교무통계학차이（P ＞0．05）。여공백대조조비교，모형대조조 SOD、MDA 수평승고，차이유통계학의의（P ＜0．05）；여모형대조조비교，장회향수제물고제량조SOD、MDA 수평화혈지강대조조 SOD 수평차이유통계학의의（P ＜0．05），여장회향수취물중、저제량조비교무통계학차이（P ＞0．05）。모형대조조여공백대조조비교 GSH-Px 유통계학차이（P ＜0．001）；수착실험적진행，장회향수제물각제량조 GSH-Px 균명현승고，모형대조조여혈지강대조조급장회향수취물고제량조비교유통계학차이（P ＜0．05），여중제량급저제량조비교무통계학차이（P ＞0．05）。결론고、중제량장회향수제물가명현조절궤체지질대사，대예방화치료고지혈증유일정적응용개치。
Objective To investigate the effect of regulation of the blood lipid and protection of liver func-tion ofCarum carvi L.in a hyperlipidemia rat model and explore its mechanism.Methods After two-week acclimation,48 10-week old male rats were divided into 6 groups randomly,including the model group, Xuezhikang group,high,middle and low doses of CCL groups (300,150,75 mg·kg-1 ·d-1 )and the normal group,8 rats in each group.All the groups except the control group were fed by a high-fat diet for 12 days and then tested the blood lipids from the tail vein.Drugs were given to the hyperlipemia rats by in-tragastric administration for 8 weeks,once daily.The model group and the normal group were given nor-mal saline instead.The body weight was measured every two weeks.The blood was collected from the ab-dominal aorta 12 hours after the last administration,high density lipoprotein cholesterol (HDL-C),low density lipoprotein cholesterol (LDL-C),total cholesterol (TC),the triglyceride (TG),blood lipid levels and aspartate amino transferase (AST),alanine transaminase (ALT)were determined in serum,lipid per-oxidase (GSH-PX)activity,superoxide dismutase (SOD)and lipid peroxide Malondialdehyde (MDA)lev-els were measured.Results Compared with the control group,TG,TC,LDL-C were significantly in-creased,and HDL-C was significantly decreased in model control group,the difference was statistically significant (P <0.05).TC,TG,LDL-C decreased,HDL-C gradually increased in each Carum carvi L.dose group (Intervention can effectively regulate lipid levels).Compared with the model group,serum lipid lev-els in each dose group were statistically significant (P <0.05).Compared with the control group,serum AST,ALT increased significantly in model control group,with significant differences (P <0.05),AST, ALT decreased significantly in Xuezhikang control group and water extract of each dose group;compared with the model group,AST,ALT were significantly different between high and middle dose groups (P <0.05-0.01),and low-dose group showed no significant difference (P >0.05).Compared with the control group,SOD,MDA levels increased in model control group,with significant differences (P <0.05);SOD, MDA levels of high-dose group and SOD level of Xuezhikan were significant compared with the model group (P <0.05).The level of GSH-Px in model control group and control group were significantly differ-ent (P < 0.05);The level of GSH-Px in model control group and Xuezhikang control group high-dose groups were significantly different (P <0.05),with the middle-dose and low-dose group showed no signif-icant difference (P >0.05).Conclusion High and middle dose of Carum carvi L.water extract can signifi-cantly regulate lipid metabolism,have a certain value on preventation and treatment of hyperlipidemia.