人卵巢上皮癌裸鼠原位移植瘤癌旁肿瘤相关基因表达及意义
인란소상피암라서원위이식류암방종류상관기인표체급의의
Expressions and significance of tumor-associated genes in tissues adjacent to human epithelial ovarian cancer of orthotopic implantation in nude mice
  • 万方数据
    中国医师杂志 중국의사잡지
    JOURNAL OF CHINESE PHYSICIAN
    2012年 11期 1445-1450 ,共6页

    朱根海%杨照新%王圣坦%蔡俊宏%陈春英%姚茂忠%洪澜%杨舒盈%丁莉莉

    주근해%양조신%왕골탄%채준굉%진춘영%요무충%홍란%양서영%정리리

    卵巢肿瘤/遗传学%卵巢肿瘤/病理学%基因表达%疾病模型,动物%小鼠,裸%肿瘤移植 卵巢腫瘤/遺傳學%卵巢腫瘤/病理學%基因錶達%疾病模型,動物%小鼠,裸%腫瘤移植
    란소종류/유전학%란소종류/병이학%기인표체%질병모형,동물%소서,라%종류이식
    Ovarian neoplasms/genetics%Ovarian neoplasms/pathology%Gene expression%Disease models,animal%Mice,nude%Neoplasm transplantation
    目的 通过对人卵巢上皮癌裸鼠原位移植瘤癌旁肿瘤相关基因表达的检测,探讨筛选癌旁正常卵巢组织的可行性.方法 人卵巢上皮癌OVCAR3细胞皮下种植获取瘤源并卵巢原位移植后,取癌组织、癌旁近端组织、癌旁中段组织、癌旁远端组织及正常裸鼠卵巢组织,采用免疫组化法检测以上组织的CK-7、CA125、P53、survivin、MMP-2、TIMP-2的表达量.结果 40例人卵巢上皮癌裸鼠原位移植瘤模型中获取35份活检正常的癌旁残余卵巢组织,获取率87.5％ (35/40).癌组织中CK-7、CA125、P53、survivin、MMP-2及TIMP-2的表达率分别为95.0％(38/40)、95.0％(38/40)、75.0％ (30/40)、85.0％ (34/40)、77.5％ (31/40)及77.5％ (31/40)；癌旁近端组织的表达率分别为71.4％ (25/35)、68.6％(24/35)、57.1％(20/35)、62.9％(22/35)、60.0％(21/35)及57.1％ (20/35)；癌旁中段组织的表达率分别为34.3％(12/35)、31.4％ (11/35)、31.4％ (11/35)、31.4％ (11/35)、34.3％ (12/35)及31.4％(11/35)；癌旁远端组织的表达率分别为25.7％ (9/35)、22.9％(8/35)、25.7％ (9/35)、25.7％ (9/35)、28.6％(10/35)及31.4％ (11/35)；23份癌旁正常卵巢组织CK-7、CA125、P53、survivin、MMP-2及TIMP-2的表达均为阴性.免疫组化检测结果为癌旁近端组织中CK-7、CA125、P53、survivin、MMP-2及TIMP-2的表达低于癌组织(P＜0.05),高于癌旁中段组织及癌旁远端组织(P＜0.01)；癌旁中段组织及癌旁远端组织表达差异无统计学意义(P＞0.05)；CK-7、CA125、survivin的强阳性表达率明显高于P53、MMP-2、TIMP-2(P＜0.01)；不同病变程度获取的癌旁正常卵巢组织中的表达量差异无统计学意义(P＞0.05).20例正常裸鼠卵巢组织CK-7、CA125、P53、survivin、MMP-2、TIMP-2的表达均为阴性.结论 CK-7、CA125、P53、survivin、MMP-2及TIMP-2等分子指标的表达向无癌方向呈递减趋势,其肿瘤相关基因表达阴性可作为筛选癌旁残余正常卵巢组织的标准,卵巢上皮癌旁可以获取相对安全的正常卵巢组织.
    목적 통과대인란소상피암라서원위이식류암방종류상관기인표체적검측,탐토사선암방정상란소조직적가행성.방법 인란소상피암OVCAR3세포피하충식획취류원병란소원위이식후,취암조직、암방근단조직、암방중단조직、암방원단조직급정상라서란소조직,채용면역조화법검측이상조직적CK-7、CA125、P53、survivin、MMP-2、TIMP-2적표체량.결과 40례인란소상피암라서원위이식류모형중획취35빈활검정상적암방잔여란소조직,획취솔87.5％ (35/40).암조직중CK-7、CA125、P53、survivin、MMP-2급TIMP-2적표체솔분별위95.0％(38/40)、95.0％(38/40)、75.0％ (30/40)、85.0％ (34/40)、77.5％ (31/40)급77.5％ (31/40)；암방근단조직적표체솔분별위71.4％ (25/35)、68.6％(24/35)、57.1％(20/35)、62.9％(22/35)、60.0％(21/35)급57.1％ (20/35)；암방중단조직적표체솔분별위34.3％(12/35)、31.4％ (11/35)、31.4％ (11/35)、31.4％ (11/35)、34.3％ (12/35)급31.4％(11/35)；암방원단조직적표체솔분별위25.7％ (9/35)、22.9％(8/35)、25.7％ (9/35)、25.7％ (9/35)、28.6％(10/35)급31.4％ (11/35)；23빈암방정상란소조직CK-7、CA125、P53、survivin、MMP-2급TIMP-2적표체균위음성.면역조화검측결과위암방근단조직중CK-7、CA125、P53、survivin、MMP-2급TIMP-2적표체저우암조직(P＜0.05),고우암방중단조직급암방원단조직(P＜0.01)；암방중단조직급암방원단조직표체차이무통계학의의(P＞0.05)；CK-7、CA125、survivin적강양성표체솔명현고우P53、MMP-2、TIMP-2(P＜0.01)；불동병변정도획취적암방정상란소조직중적표체량차이무통계학의의(P＞0.05).20례정상라서란소조직CK-7、CA125、P53、survivin、MMP-2、TIMP-2적표체균위음성.결론 CK-7、CA125、P53、survivin、MMP-2급TIMP-2등분자지표적표체향무암방향정체감추세,기종류상관기인표체음성가작위사선암방잔여정상란소조직적표준,란소상피암방가이획취상대안전적정상란소조직.
    Objective To investigate the feasibility in screening of normal ovarian tissues by evaluating the expressions of tumor-associated genes in tissues adjacent to human epithelial ovarian cancer of orthotopic implantation in nude mice.Methods Human epithelial ovarian cancer cell lines OVCAR3 were grown in subcutaneous tissues,and the tumor tissues were orthotopic implanted.The expressions of CK-7,CA125,P53,survivin,MMP-2,and TIMP-2 were detected by immunohistochemical staining in proximal tissues,middle tissues,distal tissues adjacent to tumor,tumor tissues,and normal ovarian tissues of nude mice.Results 35 samples ovarian tissues with normal biopsy were gained from 40 cases of human epithelial ovarian cancers of orthotopic implantation model in nude mice.The expression rate of CK-7,CA125,P53,survivin,MMP-2,and TIMP-2 were 95.0％ (38/40),95.0％ (38/40),75.0％ (30/40),85.0％ (34/40),77.5％ (31/40),and 77.5％ (31/40) in tumor tissues,respectively; 71.4％ (25/35),68.6％(24/35),57.1％ (20/35),62.9％ (22/35),60.0％ (21/35),and 57.1％ (20/35) in proximal tissues adjacent to tumor,respectively; 34.3％ (12/35),31.4％ (11/35),31.4％ (11/35),31.4％ (11/35),34.3％ (12/35),and 31.4％ (11/35) in middle tissues adjacent to tumor,respectively; and 25.7％ (9/35),22.9％ (8/35),25.7％ (9/35),25.7％ (9/35),28.6％(10/35),and 31.4％ (11/35) in distal tissues adjacent to tumor,re respectively.The expressions of CK-7,CA125,P53,survivin,MMP-2,and TIMP-2 were all negative in 23 samples normal ovarian tissues adjacent to tumor.The expressions of CK-7,CA125,P53,survivin,MMP-2 and TIMP-2 in proximal tissues adjacent to tumor were lower than those in tumor tissues(P＜0.05),and higher than those in middle tissues and in distal tissues adjacent to tumor(P＜0.01).There were no expression difference between in middle tissues and in distal tissues (P＞0.05).The strong positive expressions of CK-7,CA125,and survivin were higher than those of P53,MMP-2,and TIMP-2 (P＜0.01).No significant difference was found in those expressions in the normal ovarian tissues adjacent to tumor gained from orthotopic implantation model with different severity.The expressions of CK-7,CA125,P53,survivin,MMP-2,and TIMP-2 were all negative in 20 normal ovarian tissues of nude mice.Conclusions The expression of CK-7,CA125,P53,survivin,MMP-2,and TIMP-2 showed decreasing trend to non-cancer direction.Negative expressions of these tumor-associated genes can be used as standard in screening of normal ovarian tissues adjacent to tumor.Relative safe normal ovarian tissues can be obtained from the tissues adjacent to tumor.
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