中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2012年
11期
1463-1465
,共3页
叶燕乐%陈培煌%辛军%伍伯聪%黄志扬
葉燕樂%陳培煌%辛軍%伍伯聰%黃誌颺
협연악%진배황%신군%오백총%황지양
膀胱肿瘤/病理学%膀胱肿瘤/代谢%基因,肿瘤抑制%基因表达%原癌基因蛋白质类
膀胱腫瘤/病理學%膀胱腫瘤/代謝%基因,腫瘤抑製%基因錶達%原癌基因蛋白質類
방광종류/병이학%방광종류/대사%기인,종류억제%기인표체%원암기인단백질류
Urinary bladder neoplasms/pathology%Urinary bladder neoplasms/metabolism%Genes,tumor suppressor%Gene expression%Proto-oncogene proteins
目的 初步探讨膀胱尿路上皮癌中PTEN、MDM2的表达及其临床意义.方法 应用SP免疫组织化学染色法对80例膀胱尿路上皮癌及20例正常膀胱黏膜组织中PTEN、MDM2基因的表达进行检测,结合临床病理因素进行分析.结果 PTEN在不同病理分级(G1、G2、G3)和临床分期[浅表型(Tis～ T1)、浸润型(T2～T4)]的膀胱尿路上皮癌中的阳性率(86.20％、74.07％、37.50％；80.00％、46.67％)比较差异均有统计学意义(x2=15.004,P＜0.01;x2 =9.497,P＜0.01)；MDM2在不同病理分级(G1、G2、G3)和临床分期[浅表型(Tis～T1)、浸润型(T2～T4)]的膀胱尿路上皮癌中的阳性率(82.75％、55.55％、37.50％;70.00％、43.35％)比较差异均有统计学意义(x2=11.543,P ＜0.01;x2 =5.556,P＜0.05)；PTEN和MDM2在膀胱尿路上皮癌中的表达呈负相关(r=-0.611,P＜0.05).结论 PTEN和MDM2的异常表达在膀胱尿路上皮癌的发生发展中起重要作用,联合检测对于判断膀胱尿路上皮癌的恶性程度和预后有重要意义.
目的 初步探討膀胱尿路上皮癌中PTEN、MDM2的錶達及其臨床意義.方法 應用SP免疫組織化學染色法對80例膀胱尿路上皮癌及20例正常膀胱黏膜組織中PTEN、MDM2基因的錶達進行檢測,結閤臨床病理因素進行分析.結果 PTEN在不同病理分級(G1、G2、G3)和臨床分期[淺錶型(Tis～ T1)、浸潤型(T2～T4)]的膀胱尿路上皮癌中的暘性率(86.20％、74.07％、37.50％；80.00％、46.67％)比較差異均有統計學意義(x2=15.004,P＜0.01;x2 =9.497,P＜0.01)；MDM2在不同病理分級(G1、G2、G3)和臨床分期[淺錶型(Tis～T1)、浸潤型(T2～T4)]的膀胱尿路上皮癌中的暘性率(82.75％、55.55％、37.50％;70.00％、43.35％)比較差異均有統計學意義(x2=11.543,P ＜0.01;x2 =5.556,P＜0.05)；PTEN和MDM2在膀胱尿路上皮癌中的錶達呈負相關(r=-0.611,P＜0.05).結論 PTEN和MDM2的異常錶達在膀胱尿路上皮癌的髮生髮展中起重要作用,聯閤檢測對于判斷膀胱尿路上皮癌的噁性程度和預後有重要意義.
목적 초보탐토방광뇨로상피암중PTEN、MDM2적표체급기림상의의.방법 응용SP면역조직화학염색법대80례방광뇨로상피암급20례정상방광점막조직중PTEN、MDM2기인적표체진행검측,결합림상병리인소진행분석.결과 PTEN재불동병리분급(G1、G2、G3)화림상분기[천표형(Tis～ T1)、침윤형(T2～T4)]적방광뇨로상피암중적양성솔(86.20％、74.07％、37.50％；80.00％、46.67％)비교차이균유통계학의의(x2=15.004,P＜0.01;x2 =9.497,P＜0.01)；MDM2재불동병리분급(G1、G2、G3)화림상분기[천표형(Tis～T1)、침윤형(T2～T4)]적방광뇨로상피암중적양성솔(82.75％、55.55％、37.50％;70.00％、43.35％)비교차이균유통계학의의(x2=11.543,P ＜0.01;x2 =5.556,P＜0.05)；PTEN화MDM2재방광뇨로상피암중적표체정부상관(r=-0.611,P＜0.05).결론 PTEN화MDM2적이상표체재방광뇨로상피암적발생발전중기중요작용,연합검측대우판단방광뇨로상피암적악성정도화예후유중요의의.
Objective To investigate the expressions of PTEN and MDM2 in bladder transitional cell carcinoma (BTCC) and their clinical significance.Methods The expressions of PTEN and MDM2 were detected by tissue immunohistochemistry test (SP method) in BTCC (n =80) and normal bladder tissues (n =20).The relationship between PTEN and MDM2 as well as their correlations with clinical pathological features were analyzed.Results The positive rate of PTEN in different pathological grading (G1,G2,G3)and clinical staging [superficial (Tis ～ T1),infiltration (T2 ～ T4)] was (86.20％,74.07％,37.50％ ;80.00％,46.67％),respectively,with a significant difference (x2 =15.004,P ＜ 0.01 ; x2 =9.497,P ＜0.01).The positive rate of MDM2 in different pathological grading(G1,G2,G3) and clinical staging [superficial (Tis ～ T1),infiltration (T2 ～ T4)] was (82.75％,55.55％,37.50％ ; 70.00％,43.35％),respectively,with a significant differcnce(x2 =11.543,P ＜ 0.01 ; x2 =5.556,P ＜ 0.05).The expression of PTEN was negatively correlated with that of MDM2 in BTCC (r =-0.611,P ＜ 0.05).Conclusions Expressions of PTEN and MDM2 might be involved in the BTCC pathogenesis.The combined detection of PTEN and MDM2 might be of great value in the prediction of tumor behavior and prognosis.
