CAJ | 학술논문

目的：探讨黄酒对TNF-α诱导的大鼠血管内皮功能的影响。方法大鼠原代主动脉血管内皮细胞（VECs）经分离培养及纯化鉴定后，取第3～4代细胞用于实验。不同浓度酒精（1.0%、1.2%、1.4%、1.6%、1.8%、2.0%）与50μg/L TNF-α共同孵育大鼠 VECs）48h，MTT 法检测酒类对细胞活性的影响，确定最佳干预浓度后分为对照组、TNF-α组、TNF-α+瑞舒他汀组（10μmol/L）、TNF-α+酒精组（0.5%、1.0%、1.5%）、TNF-α+黄酒组（0.5%、1.0%、1.5%），共9组。培养24h后收集样品，硝酸还原酶法测定培养液上清液NO的含量，化学比色法测定血浆中内皮型一氧化氮合酶（eNOS）活性，免疫印迹法检测VECs中eNOS、细胞间黏附分子-1（ICAM-1）的表达量。结果与TNF-α组相比，瑞舒伐他汀组、黄酒1.0%组、黄酒1.5%组eNOS活力、eNOS的表达及NO含量升高（P＜0.01或0.05），ICAM-1表达降低（P＜0.01或0.05）；与瑞舒伐他汀组相比，黄酒1.0%组及黄酒1.5%组eNOS表达降低，ICAM-1表达升高（P＜0.01或0.05）。结论小剂量黄酒能够增强eNOS的活力及其表达，可使NO含量增加，抑制ICAM-1表达，具有类他汀样作用。
목적：탐토황주대TNF-α유도적대서혈관내피공능적영향。방법대서원대주동맥혈관내피세포（VECs）경분리배양급순화감정후，취제3～4대세포용우실험。불동농도주정（1.0%、1.2%、1.4%、1.6%、1.8%、2.0%）여50μg/L TNF-α공동부육대서 VECs）48h，MTT 법검측주류대세포활성적영향，학정최가간예농도후분위대조조、TNF-α조、TNF-α+서서타정조（10μmol/L）、TNF-α+주정조（0.5%、1.0%、1.5%）、TNF-α+황주조（0.5%、1.0%、1.5%），공9조。배양24h후수집양품，초산환원매법측정배양액상청액NO적함량，화학비색법측정혈장중내피형일양화담합매（eNOS）활성，면역인적법검측VECs중eNOS、세포간점부분자-1（ICAM-1）적표체량。결과여TNF-α조상비，서서벌타정조、황주1.0%조、황주1.5%조eNOS활력、eNOS적표체급NO함량승고（P＜0.01혹0.05），ICAM-1표체강저（P＜0.01혹0.05）；여서서벌타정조상비，황주1.0%조급황주1.5%조eNOS표체강저，ICAM-1표체승고（P＜0.01혹0.05）。결론소제량황주능구증강eNOS적활력급기표체，가사NO함량증가，억제ICAM-1표체，구유류타정양작용。
Objective To study the rice wine whether its effects are similar to statin and to study the possibility that rice wine inhibit the production of Tumor Necrosis Factor- α(TNF- α)- induced endothelialnitricoxidesynthase(eNOS),nitric of rice ox-ide(NO)and intercellularadhesionmolecule- 1(ICAM- 1) in cultured rat vascular endothelial cel s(VECs). Methods Isolation,culti-vation,purification and identification of VECs of rat thoracic aorta in vitro were conducted. The VECs in passages 3 and 4 were used in al studies. The VECs were incubated with one kind of wine (at the concentrations of 1.0%,1.2%,1.4%,1.6%,1.8%,2.0%) and 50ug/L TNF- αfor 48h.The optimal concentration of wine was selected. In another experiment, the cel s were divided into 9 groups:control, TNF- α,TNF- α+rosuvastatin (10μlmol/L)、TNF- α+ethanol (0.5%、1.0%、1.5%)、TNF- α+rice wine (0.5%、1.0%、1.5%), and were given the corresponding treatment for 24h.Theproductionof NO was determined with nitratereductase. The activ-ity of eNOS was then of nitric oxide (NO) was determined with nitratereductase. The activity of eNOS was then measured by means of chemicalchromatometry.The protein level of eNOS and ICAM- 1 were detected by Western blotting. Results Com-pared with TNF- α group, the activity of eNOS, the protein expression of eNOS and the production of NO in rosuvastatin, rice wine 1.0%or rice wine 1.5%group were significantly increased.The protein expression of ICAM- 1 in rosuvastatin, rice wine 1.0%or rice wine 1.5%group was significantly decreased yet. As wel , compared with rosuvastatin, the protein expression of eNOS in rice wine 1.0%or rice wine 1.5%group was significantly decreased. The protein expression of ICAM- 1 in rice wine 1.0%or rice wine 1.5% group was significantly increased. Conclusion Treatment with rice wine increases the activity of eNOS, the protein expression of eNOS and the production of NO, which decreases the protein expression of ICAM- 1.