CAJ | 학술논문

目的研究Pax-8基因敲除小鼠心肌细胞中差异表达的基因。方法利用基因芯片技术初筛在Pax-8基因敲除纯合子小鼠（Pax-8-/-）和杂合子小鼠（Pax-8+/-）心肌组织中差异表达的基因，运用半定量RT- PCR检验初筛结果，并运用荧光实时定量PCR确定初筛所得的差异表达的基因在Pax-8基因敲除纯合子、杂合子以及野生型小鼠（Pax-8+/+）心肌组织中的表达情况。结果基因Bcl2l14、Fgf-6、Lck、Nr4a1以及Tcf7在Pax-8-/-中的表达分别是其在Pax-8+/-中表达的2.07±0.08、1.13±0.01、1.30±0.01、1.53±0.08及0.75±0.03倍（均P＜0.01）。是在Pax-8+/+中表达的2.23±0.16、1.67±0.05、1.77±0.21、4.8±0.35及0.9±0.04倍（P＜0.01或0.05）。结论 Bcl2l14、Fgf-6、Lck、Nr4a1以及Tcf7上述5个基因在Pax-8基因敲除的心肌细胞中差异表达，参与Pax-8基因敲除小鼠的一系列心脏病理生理变化。
목적연구Pax-8기인고제소서심기세포중차이표체적기인。방법이용기인심편기술초사재Pax-8기인고제순합자소서（Pax-8-/-）화잡합자소서（Pax-8+/-）심기조직중차이표체적기인，운용반정량RT- PCR검험초사결과，병운용형광실시정량PCR학정초사소득적차이표체적기인재Pax-8기인고제순합자、잡합자이급야생형소서（Pax-8+/+）심기조직중적표체정황。결과기인Bcl2l14、Fgf-6、Lck、Nr4a1이급Tcf7재Pax-8-/-중적표체분별시기재Pax-8+/-중표체적2.07±0.08、1.13±0.01、1.30±0.01、1.53±0.08급0.75±0.03배（균P＜0.01）。시재Pax-8+/+중표체적2.23±0.16、1.67±0.05、1.77±0.21、4.8±0.35급0.9±0.04배（P＜0.01혹0.05）。결론 Bcl2l14、Fgf-6、Lck、Nr4a1이급Tcf7상술5개기인재Pax-8기인고제적심기세포중차이표체，삼여Pax-8기인고제소서적일계렬심장병리생리변화。
Objective To investigate differential y expressed genes in myocardium of Pax- 8 gene knockout mice. Methods Candidate Pax- 8 downstream genes were screening by mouse genome DNA microarray with Pax- 8-/- and Pax- 8+/-cDNA, then tested by semi- quantitative RT PCR. Real time RT- PCR was performed to confirm the differential y expressed down-stream genes. Results The expression of genes Bcl2l14, Fgf- 6, Lck, Nr4a1 and Tcf7 in Pax- 8-/- mice was 2.07±0.08, 1.13± 0.01, 1.3±0.01, 1.53±0.08, 0.75±0.03 times as expressed in Pax- 8+/- mice (P<0.01), and 2.23±0.16, 1.67±0.05, 1.77±0.21, 4.8±0.35 (P<0.01), 0.9±0.04 (P<0.05) times as expressed in Pax- 8+/+mice, respectively. Conclusion Genes Bcl2l14, Fgf- 6, Lck, Nr4a1 and Tcf7 were differential y expressed in myocardium of Pax- 8 gene knockout mice, which might be involved in a se-ries of pathophysiologic changes in the gene knockout myocardium.