中国骨质疏松杂志
中國骨質疏鬆雜誌
중국골질소송잡지
CHINESE JOURNAL OF OSTEOPOROSIS
2014年
8期
913-919
,共7页
邓伟民%张金赫%刘泽%张亚松%康庐琛
鄧偉民%張金赫%劉澤%張亞鬆%康廬琛
산위민%장금혁%류택%장아송%강려침
5-羟色胺%骨密度%补肾壮骨冲剂%阿仑膦酸钠
5-羥色胺%骨密度%補腎壯骨遲劑%阿崙膦痠鈉
5-간색알%골밀도%보신장골충제%아륜련산납
5-Serotonin%Bone mineral density%Bushenzhuanggu granules%Alendronate sodium
目的:观察补肾壮骨冲剂和阿仑膦酸钠对骨质疏松症(osteoporosis,OP)患者的肠源性5-羟色胺(5-hydroxytryptamine,5-HT)浓度和骨密度的影响,探讨其作用机制。方法将120例原发性骨质疏松症患者分为补肾壮骨冲剂组和阿仑膦酸钠组,分析在药物治疗前后肠源性5-HT浓度和骨密度的差异。(1)本研究所有样本为2011年5月-2012年12月到广州军区广州总医院住院部及门诊部就诊的确诊为原发性骨质疏松症的患者。(2)肠源性5-HT测定:上午8:00~9:00抽取禁食12 h后空腹肘静脉血3 ml,3000 r/min即时离心5 min后,将血清置于-80℃冰箱保存,采用酶联免疫分析法检测5-HT的含量。(3)骨密度测定:采用美国GE公司生产的Lunar Prodigy双能X线(DEXA)骨密度仪,检测各部位BMD。(4)骨生化指标测定:采用酶联免疫分析法检测血清中肠源性5-HT的含量、电化学发光免疫分析法分析血清P1NP、β-Crosslaps、PTH的含量。结果补肾壮骨冲剂组治疗后左侧股骨颈、Ward’ s三角和左侧股骨近端处骨密度上升,血清中的5-HT、PINP、β-Crosslaps及PTH浓度全面下降。阿仑膦酸钠组治疗后除腰椎外,其余各部位的骨密度都明显上升,血清中的5-HT、PINP、β-Crosslaps及PTH浓度全面下降。补肾壮骨冲剂组与阿仑膦酸钠组治疗后相关指标比较,两组骨密度改善情况未见明显差别,两组在治疗后的各项骨代谢指标改善情况未见明显差别。结论补肾壮骨冲剂和阿仑膦酸钠均可在提高骨密度同时减低肠源性5-HT浓度,与Ulrike提出的“肠源性5-HT与骨密度成负相关”这个结论相符。
目的:觀察補腎壯骨遲劑和阿崙膦痠鈉對骨質疏鬆癥(osteoporosis,OP)患者的腸源性5-羥色胺(5-hydroxytryptamine,5-HT)濃度和骨密度的影響,探討其作用機製。方法將120例原髮性骨質疏鬆癥患者分為補腎壯骨遲劑組和阿崙膦痠鈉組,分析在藥物治療前後腸源性5-HT濃度和骨密度的差異。(1)本研究所有樣本為2011年5月-2012年12月到廣州軍區廣州總醫院住院部及門診部就診的確診為原髮性骨質疏鬆癥的患者。(2)腸源性5-HT測定:上午8:00~9:00抽取禁食12 h後空腹肘靜脈血3 ml,3000 r/min即時離心5 min後,將血清置于-80℃冰箱保存,採用酶聯免疫分析法檢測5-HT的含量。(3)骨密度測定:採用美國GE公司生產的Lunar Prodigy雙能X線(DEXA)骨密度儀,檢測各部位BMD。(4)骨生化指標測定:採用酶聯免疫分析法檢測血清中腸源性5-HT的含量、電化學髮光免疫分析法分析血清P1NP、β-Crosslaps、PTH的含量。結果補腎壯骨遲劑組治療後左側股骨頸、Ward’ s三角和左側股骨近耑處骨密度上升,血清中的5-HT、PINP、β-Crosslaps及PTH濃度全麵下降。阿崙膦痠鈉組治療後除腰椎外,其餘各部位的骨密度都明顯上升,血清中的5-HT、PINP、β-Crosslaps及PTH濃度全麵下降。補腎壯骨遲劑組與阿崙膦痠鈉組治療後相關指標比較,兩組骨密度改善情況未見明顯差彆,兩組在治療後的各項骨代謝指標改善情況未見明顯差彆。結論補腎壯骨遲劑和阿崙膦痠鈉均可在提高骨密度同時減低腸源性5-HT濃度,與Ulrike提齣的“腸源性5-HT與骨密度成負相關”這箇結論相符。
목적:관찰보신장골충제화아륜련산납대골질소송증(osteoporosis,OP)환자적장원성5-간색알(5-hydroxytryptamine,5-HT)농도화골밀도적영향,탐토기작용궤제。방법장120례원발성골질소송증환자분위보신장골충제조화아륜련산납조,분석재약물치료전후장원성5-HT농도화골밀도적차이。(1)본연구소유양본위2011년5월-2012년12월도엄주군구엄주총의원주원부급문진부취진적학진위원발성골질소송증적환자。(2)장원성5-HT측정:상오8:00~9:00추취금식12 h후공복주정맥혈3 ml,3000 r/min즉시리심5 min후,장혈청치우-80℃빙상보존,채용매련면역분석법검측5-HT적함량。(3)골밀도측정:채용미국GE공사생산적Lunar Prodigy쌍능X선(DEXA)골밀도의,검측각부위BMD。(4)골생화지표측정:채용매련면역분석법검측혈청중장원성5-HT적함량、전화학발광면역분석법분석혈청P1NP、β-Crosslaps、PTH적함량。결과보신장골충제조치료후좌측고골경、Ward’ s삼각화좌측고골근단처골밀도상승,혈청중적5-HT、PINP、β-Crosslaps급PTH농도전면하강。아륜련산납조치료후제요추외,기여각부위적골밀도도명현상승,혈청중적5-HT、PINP、β-Crosslaps급PTH농도전면하강。보신장골충제조여아륜련산납조치료후상관지표비교,량조골밀도개선정황미견명현차별,량조재치료후적각항골대사지표개선정황미견명현차별。결론보신장골충제화아륜련산납균가재제고골밀도동시감저장원성5-HT농도,여Ulrike제출적“장원성5-HT여골밀도성부상관”저개결론상부。
Objective To observe the effect of the nourishing kidney and strengthening bone granule and alendronate on the changes of enterogenous 5-hydroxytryptamine (5-HT) and bone mineral density ( BMD) in patients with osteoporosis ( OP) , and to explore its mechanism.Methods One hundred and tewenty patients with primary OP, who came to the inpatient and outpatient department in the Genenral Hospital of PLA Guangzhou Military Area from May 2011 to December 2012, were selected.All the patients were divided into the nourishing kidney and strengthening bone granule group and alendronate group.After 12-hour fasting, 3ml fasting venous blood of each patient was collected during 8:00-9:00 am.The blood was instantly centrifuged (3000 r/min) for 5 min, and the serum was stored in a -80℃ refrigerator.The concentration of 5-HT in the serum was determined using ELISA. BMD of each part was detected using the dual energy X-rayabsorptiometry ( DEXA, Lunar Prodigy, GE Co., USA) .The changes of 5-HT and BMD before and after the treatment were analyzed.The serum concentrations of P1NP,β-Crosslaps, and PTH were detected using electrochemiluminescence immunoassay.Results BMD of the left femoral neck, the Ward’ s triangle, and the left proximal femur increased in the the nourishing kidney and strengthening bone granule group after the treatment, while the serum levels of 5-HT, PINP,β-Crosslaps, and PTH decreased.In alendronate group, except for BMD of the lumbar vertebrae, BMD of the rest parts increased obviously after the treatment.And the serum levels of 5-HT, PINP,β-Crosslaps, and PTH also decreased. According to the comparison of the related indexes between the nourishing kidney and strengthening bone granule group and alendronate group after the treatment, no significant difference of BMD between the two groups was observed.The changes of the bone metabolic indexes between the two groups before and after treatment also showed no significant differenc.Conclusion The nourishing kidney and strengthening bone granule and alendronate sodium can both increase BMD and reduce the enterogenous 5-HT concentration, and it is coincide with Ulrike’ s theory that gut-derived 5-HT was negatively related with BMD.