CAJ | 학술논문

目的：观察淫羊藿苷对体外培养小鼠破骨细胞MMP－9、CK mRNA表达的影响。方法用浓度分别为25 ng／ml、30 ng／ml、10－8 mol／L的巨噬细胞集落刺激因子（M－CSF）、核因子κB受体活化因子配体（RANKL）、1，25（OH）2VitD3体外诱导培养小鼠骨髓源性破骨细胞，分别加入0 mol／L、10－5 mol／L的淫羊藿苷，培养48 h后，抽提总RNA，采用半定量逆转录－聚合酶链反应（RT－PCR）方法检测破骨细胞中基质金属蛋白酶－9（MMP－9）、组织蛋白酶 K （CK） mRNA表达的变化。结果与空白对照组比较，10－5 mol／L的淫羊藿苷可明显下调MMP9mRNA的表达（P＜0．05），但对CK mRNA的表达无明显影响（P＞0．05）。结论淫羊藿可以通过下调MMP－9基因表达，从而抑制破骨细胞的分化形成及骨吸收。
목적：관찰음양곽감대체외배양소서파골세포MMP－9、CK mRNA표체적영향。방법용농도분별위25 ng／ml、30 ng／ml、10－8 mol／L적거서세포집락자격인자（M－CSF）、핵인자κB수체활화인자배체（RANKL）、1，25（OH）2VitD3체외유도배양소서골수원성파골세포，분별가입0 mol／L、10－5 mol／L적음양곽감，배양48 h후，추제총RNA，채용반정량역전록－취합매련반응（RT－PCR）방법검측파골세포중기질금속단백매－9（MMP－9）、조직단백매 K （CK） mRNA표체적변화。결과여공백대조조비교，10－5 mol／L적음양곽감가명현하조MMP9mRNA적표체（P＜0．05），단대CK mRNA적표체무명현영향（P＞0．05）。결론음양곽가이통과하조MMP－9기인표체，종이억제파골세포적분화형성급골흡수。
Objective To observe the effect of icariin on the expression of MMP-9 and CK mRNA in cultured mouse osteoclasts in vitro.Methods Osteoclasts were obtained from mouse bone marrow and cultured with M-CSF (25ng/ml), RANKL (30ng/ml), and 1,25-(OH)2VitD3(10-8mol/L).After treated with different concentrations of icariin (0mol/L, 10 -5mol/L) for 48h, total RNA was isolated from osteoclasts.The expression of metalloproteinase-9 ( MMP-9) and cathepsin K ( CK) mRNA was detected using semi-quantitative RT-PCR.Results Compared with that in the control group, the expression of MMP-9 mRNA in 10 -5 mol/L icariin group remarkably decreased ( P <0.05 ) , but no significant effect on the expression of CK mRNA was observed ( P >0.05 ) . Conclusion Icariin can inhibit the differentiation of osteoclasts and bone resorption via down-regulating the expression of MMP-9 mRNA.