临床肾脏病杂志
臨床腎髒病雜誌
림상신장병잡지
JOURNAL OF CLINICAL NEPHROLOGY
2014年
10期
626-630
,共5页
硫酸吲哚酚%血管平滑肌细胞%增生%辛伐他汀%氧化应激
硫痠吲哚酚%血管平滑肌細胞%增生%辛伐他汀%氧化應激
류산신타분%혈관평활기세포%증생%신벌타정%양화응격
Indoxyl sulfate%Vascular smooth muscle cells%Proliferation%Simvastatin%Oxida-tive stress
目的：探讨硫酸吲哚酚(indoxyl sulfate,IS)对大鼠血管平滑肌细胞(vascular smooth muscle cell,VSMC)增生的影响以及这种变化和氧化应激的关系。方法实验均分为8组：正常组、IS 100μM组、IS 300μM组、IS 500μM组、辛伐他汀10μM+正常组、辛伐他汀10μM+IS 100μM组、辛伐他汀10μM+IS 300μM组、辛伐他汀10μM+IS 500μM组。采用WST-1法检测VSMC增殖情况；硫代巴比妥酸法检测培养基中丙二醛(malondialdehyde,MDA)含量；ELISA方法测定晚期氧化蛋白产物(advanced oxidation protein products,AOPP)。结果与正常组比较,IS 300μM组和 IS 500μM 组上清液的 WST-1(OD 值)[(1.55±0.27)比(1.18±0.25)与(1.73±0.30)比(1.18±0.25),P<0.01]含量、MDA含量[(2.60±0.47)μg/ml比(1.59±0.21)μg/ml与(2.82±0.54)μg/ml比(1.59±0.21)μg/ml,P<0.01]和 AOPP 含量[(67.94±8.58)μmol/L 比(54.97±8.46)μmol/L与(72.09±9.49)μmol/L比(54.97±8.46)μmol/L,P<0.01]均明显增高。与同浓度 IS 组相比,辛伐他汀10μM+IS 300μM 组和辛伐他汀10μM+IS 500μM 组上清液 WST-1(OD 值)[(1.22±0.24)比(1.55±0.27)与(1.32±0.30)比(1.73±0.30),P<0.05]、MDA[(1.64±0.38)μg/mL 比(2.60±0.47)μg/ml 与(1.70±0.40)μg/ml 比(2.82±0.54)μg/ml,P<0.01]含量和 AOPP 含量[(55.56±7.41)μmol/L比(67.94±8.58)μmol/L 与(55.54±6.80)μmol/L 比(72.09±9.49)μmol/L,P<0.05]均明显降低。大鼠 VSMC 上清液的 WST-1含量与 MDA 含量呈正相关(r=0.621,P<0.01),大鼠VSMC上清液的WST-1含量与AOPP含量呈正相关(r=0.581,P<0.01)。结论 IS可浓度依赖性地促进大鼠 VSMC增生,其作用可能与 IS增加 VSMC的氧化应激有关；辛伐他汀可抑制此作用。
目的：探討硫痠吲哚酚(indoxyl sulfate,IS)對大鼠血管平滑肌細胞(vascular smooth muscle cell,VSMC)增生的影響以及這種變化和氧化應激的關繫。方法實驗均分為8組：正常組、IS 100μM組、IS 300μM組、IS 500μM組、辛伐他汀10μM+正常組、辛伐他汀10μM+IS 100μM組、辛伐他汀10μM+IS 300μM組、辛伐他汀10μM+IS 500μM組。採用WST-1法檢測VSMC增殖情況；硫代巴比妥痠法檢測培養基中丙二醛(malondialdehyde,MDA)含量；ELISA方法測定晚期氧化蛋白產物(advanced oxidation protein products,AOPP)。結果與正常組比較,IS 300μM組和 IS 500μM 組上清液的 WST-1(OD 值)[(1.55±0.27)比(1.18±0.25)與(1.73±0.30)比(1.18±0.25),P<0.01]含量、MDA含量[(2.60±0.47)μg/ml比(1.59±0.21)μg/ml與(2.82±0.54)μg/ml比(1.59±0.21)μg/ml,P<0.01]和 AOPP 含量[(67.94±8.58)μmol/L 比(54.97±8.46)μmol/L與(72.09±9.49)μmol/L比(54.97±8.46)μmol/L,P<0.01]均明顯增高。與同濃度 IS 組相比,辛伐他汀10μM+IS 300μM 組和辛伐他汀10μM+IS 500μM 組上清液 WST-1(OD 值)[(1.22±0.24)比(1.55±0.27)與(1.32±0.30)比(1.73±0.30),P<0.05]、MDA[(1.64±0.38)μg/mL 比(2.60±0.47)μg/ml 與(1.70±0.40)μg/ml 比(2.82±0.54)μg/ml,P<0.01]含量和 AOPP 含量[(55.56±7.41)μmol/L比(67.94±8.58)μmol/L 與(55.54±6.80)μmol/L 比(72.09±9.49)μmol/L,P<0.05]均明顯降低。大鼠 VSMC 上清液的 WST-1含量與 MDA 含量呈正相關(r=0.621,P<0.01),大鼠VSMC上清液的WST-1含量與AOPP含量呈正相關(r=0.581,P<0.01)。結論 IS可濃度依賴性地促進大鼠 VSMC增生,其作用可能與 IS增加 VSMC的氧化應激有關；辛伐他汀可抑製此作用。
목적：탐토류산신타분(indoxyl sulfate,IS)대대서혈관평활기세포(vascular smooth muscle cell,VSMC)증생적영향이급저충변화화양화응격적관계。방법실험균분위8조：정상조、IS 100μM조、IS 300μM조、IS 500μM조、신벌타정10μM+정상조、신벌타정10μM+IS 100μM조、신벌타정10μM+IS 300μM조、신벌타정10μM+IS 500μM조。채용WST-1법검측VSMC증식정황；류대파비타산법검측배양기중병이철(malondialdehyde,MDA)함량；ELISA방법측정만기양화단백산물(advanced oxidation protein products,AOPP)。결과여정상조비교,IS 300μM조화 IS 500μM 조상청액적 WST-1(OD 치)[(1.55±0.27)비(1.18±0.25)여(1.73±0.30)비(1.18±0.25),P<0.01]함량、MDA함량[(2.60±0.47)μg/ml비(1.59±0.21)μg/ml여(2.82±0.54)μg/ml비(1.59±0.21)μg/ml,P<0.01]화 AOPP 함량[(67.94±8.58)μmol/L 비(54.97±8.46)μmol/L여(72.09±9.49)μmol/L비(54.97±8.46)μmol/L,P<0.01]균명현증고。여동농도 IS 조상비,신벌타정10μM+IS 300μM 조화신벌타정10μM+IS 500μM 조상청액 WST-1(OD 치)[(1.22±0.24)비(1.55±0.27)여(1.32±0.30)비(1.73±0.30),P<0.05]、MDA[(1.64±0.38)μg/mL 비(2.60±0.47)μg/ml 여(1.70±0.40)μg/ml 비(2.82±0.54)μg/ml,P<0.01]함량화 AOPP 함량[(55.56±7.41)μmol/L비(67.94±8.58)μmol/L 여(55.54±6.80)μmol/L 비(72.09±9.49)μmol/L,P<0.05]균명현강저。대서 VSMC 상청액적 WST-1함량여 MDA 함량정정상관(r=0.621,P<0.01),대서VSMC상청액적WST-1함량여AOPP함량정정상관(r=0.581,P<0.01)。결론 IS가농도의뢰성지촉진대서 VSMC증생,기작용가능여 IS증가 VSMC적양화응격유관；신벌타정가억제차작용。
Objective To investigate the effect of indoxyl sulfate (IS)on proliferation of rat vascular smooth muscle cells (VSMCs)and the relationship between oxidative stress and this effect. Methods Cultured rat VSMCs were divided into normal group,100μM IS treatment group,300μM IS treatment group,500μM IS treatment group,simvastatin10μM+normal saline group,simvastatin 10μM+100μM IS treatment group,simvastatin 10μM+300μM IS treatment group and simvastatin 10μM+500μM IS treatment group.Calcium deposition in VSMCs was measured by BCA.The cell proliferation in VSMCs was detected by WST-1 method.Themalondialdehyde (MDA)content was de-termined by thiobarbituric acid method.Advanced oxidation protein products (AOPP)content was ex-amined by ELISA.Results As compared with normal group,the WST-1 contents in the supernatant of 300μM IS treatment group and 500μM IS treatment group [A values of (1.55±0.27)and (1.73 ±0.30)vs.(1.18±0.25)(P<0.01)],MDA contents [(2.60±0.47)and (2.82±0.54)μg/mL vs.(1.59±0.21)μg/mL (P<0.01)],and AOPP contents [(67.94±8.58)and (72.09±9.49)μmol/L vs.(54.97±8.46)μmol/L (P<0.01)]were significantly increased.As compared with the same con-centration IS treatment group,the WST-1 contents in the supernatant of simvastatin 10μM+300μM IS treatment group and simvastatin 10μM+500μM IS treatment group [A values of (1.22±0.24) vs.(1.55 ±0.27)(P<0.05)and (1.32 ±0.30)vs.(1.73 ±0.30)(P<0.05)],MDA contents [(1.64±0.38)vs.(2.60±0.47)μg/mL (P<0.01)and (1.70±0.40)vs.(2.82±0.54)μg/mL (P<0.01)],and AOPP contents [(55.56±7.41)vs.(67.94±8.58)μmol/L (P<0.05)and (55.54 ±6.80)vs.(72.09±9.49)μmol/L (P<0.05)]were significantly decreased.There was significantly positive correlation between the WST-1 contents and MDA contents (r=0.621,P<0.01),and be-tween the WST-1 contents and AOPP contents (r=0.581,P<0.01).Conclusions IS may promote proliferation of rat VSMCs in a concentration-dependent manner,which may be possibly related with the increased oxidative stress induced by IS.Simvastatin may inhibit this effect.
