CAJ | 학술논문

目的：检测同源盒基因B7（ HOXB7）在肺腺癌中的表达情况，并探讨其临床意义。方法分别应用逆转录PCR（ RT?PCR）和Western blotting法检测人正常肺支气管上皮细胞系HBE及4株肺腺癌细胞系A549、H1975、H1650、H322中HOXB7的表达情况。应用免疫组织化学染色及Western blotting法检测HOXB7在75例肺腺癌及配对癌旁正常组织中的表达，并分析其表达与肺腺癌临床病理特征及预后的关系。结果4株肺腺癌细胞系中HOXB7 mRNA和蛋白的表达水平均显著高于正常肺支气管上皮细胞系（P＜0?05）。 HOXB7蛋白在肺腺癌及对应癌旁正常组织中的高表达率分别为76?0％（57／75）和9?3％（7／75），差异有统计学意义（ P＜0?05）。 HOXB7蛋白在肺腺癌组织中的表达水平与临床分期、病理分级及淋巴结转移有关（ P＜0?05），而与性别、年龄无关（ P＞0?05）。 HOXB7低表达者的中位生存期为68个月，高于高表达者的51个月，差异有统计学意义（ P＜0?05）。结论 HOXB7在肺腺癌中高表达，且与肿瘤的侵袭转移和疾病进展相关，有可能作为肺腺癌早期诊断及评估预后的新靶标。
목적：검측동원합기인B7（ HOXB7）재폐선암중적표체정황，병탐토기림상의의。방법분별응용역전록PCR（ RT?PCR）화Western blotting법검측인정상폐지기관상피세포계HBE급4주폐선암세포계A549、H1975、H1650、H322중HOXB7적표체정황。응용면역조직화학염색급Western blotting법검측HOXB7재75례폐선암급배대암방정상조직중적표체，병분석기표체여폐선암림상병리특정급예후적관계。결과4주폐선암세포계중HOXB7 mRNA화단백적표체수평균현저고우정상폐지기관상피세포계（P＜0?05）。 HOXB7단백재폐선암급대응암방정상조직중적고표체솔분별위76?0％（57／75）화9?3％（7／75），차이유통계학의의（ P＜0?05）。 HOXB7단백재폐선암조직중적표체수평여림상분기、병리분급급림파결전이유관（ P＜0?05），이여성별、년령무관（ P＞0?05）。 HOXB7저표체자적중위생존기위68개월，고우고표체자적51개월，차이유통계학의의（ P＜0?05）。결론 HOXB7재폐선암중고표체，차여종류적침습전이화질병진전상관，유가능작위폐선암조기진단급평고예후적신파표。
Objective To detect the expression of homeobox genes( HOX) B7 in human lung adenocarcinoma, and investigate its clinical significance. Methods The expressions of HOXB7 mRNA and proteins in human lung adenocarcinoma cell lines A549, H1975, H1650, H322 and human bronchial epithelial cell line HBE were detected by reverse transcription PCR( RT?PCR) and West?ern blotting method, respectively. Immunohistochemistry staining was used to examine the expressions of HOXB7 in 75 samples of lung adenocarcinoma and their corresponding adjacent normal lung tissues. The correlation between HOXB7 expression and clinicopathological features and prognosis of lung adenocarcinoma were analyzed. Results The expression levels of HOXB7 mRNA and proteins in four lung adenocarcinoma cell lines were significantly higher than those in human bronchial epithelial cell line. The over?ex?pression rate of HOXB7 in lung adenocarcinoma and corresponding paracancerous tissue was 76?0%( 57/75) and 9?3%( 7/75) with statistical difference( P<0?05) . Statistical analysis revealed that HOXB7 protein expression was correlated with clinical stage, patholog?ical grade and lymph node metastasis in lung adenocarcinoma( P<0?05) . The median overall survival time of patients with low HOXB7 expression was 68 months, higher than 51 months of patients with over?expression with statistically significant( P<0?05) . Conclusion The over?expression of HOXB7 relates to the invasion, metastasis and progression of lung adenocarcinoma. It may play an important role in earlier diagnosis and prognosis evaluation for lung adenocarcinoma.