CAJ | 학술논문

目的：检测Ⅲ期肺腺癌组织中14?3?3ζ和E?钙黏蛋白（ E?cadherin）的表达，并探讨其临床意义。方法采用免疫组化SP法检测59例Ⅲ期肺腺癌组织和43例正常肺组织中14?3?3ζ与E?cadherin蛋白的表达水平，分析两者表达的相关性以及与临床病理特征和预后的关系。结果14?3?3ζ在Ⅲ期肺腺癌组织中的高表达率为57?63％（34／59），高于正常肺组织的30?23％（13／43），差异有统计学意义（P＜0?05）；E?cadherin在肺腺癌组织中的高表达率为67?79％（40／59），低于正常肺组织的72?09％（31／43），差异无统计学意义（P＞0?05）。2种蛋白的表达均与肺腺癌的分化程度相关（P＜0?05）。Ⅲ期肺腺癌中14?3?3ζ与E?cadherin的表达呈显著正相关（ r＝0?351，P＝0?015）。全组患者的中位生存时间（ OS）为29?90个月。14?3?3ζ、E?cadherin高表达患者的中位OS均为52?63个月，优于低表达者的16?93个月（ P＜0?05）。 Cox多因素分析显示，14?3?3ζ是Ⅲ期肺腺癌预后的独立影响因素（ HR＝0?345，95％CI：0?153～0?777，P＝0?010）。结论14?3?3ζ与E?cadherin在Ⅲ期肺腺组织中的表达呈现较好的相关性，检测二者的表达对判断Ⅲ期肺腺癌患者的预后有一定提示作用。
목적：검측Ⅲ기폐선암조직중14?3?3ζ화E?개점단백（ E?cadherin）적표체，병탐토기림상의의。방법채용면역조화SP법검측59례Ⅲ기폐선암조직화43례정상폐조직중14?3?3ζ여E?cadherin단백적표체수평，분석량자표체적상관성이급여림상병리특정화예후적관계。결과14?3?3ζ재Ⅲ기폐선암조직중적고표체솔위57?63％（34／59），고우정상폐조직적30?23％（13／43），차이유통계학의의（P＜0?05）；E?cadherin재폐선암조직중적고표체솔위67?79％（40／59），저우정상폐조직적72?09％（31／43），차이무통계학의의（P＞0?05）。2충단백적표체균여폐선암적분화정도상관（P＜0?05）。Ⅲ기폐선암중14?3?3ζ여E?cadherin적표체정현저정상관（ r＝0?351，P＝0?015）。전조환자적중위생존시간（ OS）위29?90개월。14?3?3ζ、E?cadherin고표체환자적중위OS균위52?63개월，우우저표체자적16?93개월（ P＜0?05）。 Cox다인소분석현시，14?3?3ζ시Ⅲ기폐선암예후적독립영향인소（ HR＝0?345，95％CI：0?153～0?777，P＝0?010）。결론14?3?3ζ여E?cadherin재Ⅲ기폐선조직중적표체정현교호적상관성，검측이자적표체대판단Ⅲ기폐선암환자적예후유일정제시작용。
Objective To investigate the expressions of 14?3?3ζand E?cadherin in resected stageⅢlung adenocarcinoma and their clinical significance. Methods The expressions of 14?3?3ζand E?cadherin in resected stageⅢlung adenocarcinoma tissues ob?tained from 59 patients and 43 specimens of corresponding adjacent normal alveolus tissues using immunohistochemistry,and their cor?relations with clinicopathological characteristics and prognosis of patients were analyzed. The overall survival( OS) of patients was esti?mated using the Kaplan?Meier method. The factors affecting the prognosis of stage Ⅲ lung adenocarcinoma patients were analyzed by Cox multivariate survival analysis. Results The high?level expression rate of 14?3?3ζin lung adenocarcinoma tissue was 57?63%( 34/59) , higher than 30?23( 13/43) of paired adjacent normal alveolus tissues with statistical difference( P<0?05) . The high?level expres?sion rate of E?cadherin in lung adenocarcinoma tissue was 67?79%(40/59), lower than 72?09%(31/43) of paired adjacent normal alveolus tissues without statistical difference( P>0?05) . Both of 14?3?3ζand E?cadherin expressions were correlated to differentiation( P<0?05).Positive correlation was observed between expressions of 14?3?3ζand E?cadherin (r=0?351,P=0?015).The median OS of 59 patients was 29?90 months. The median OS of patients with 14?3?3ζ and E?cadherin high?level expression both were 52?63 months, higher than 16?93 months of patients with low?level expression( P<0?05) . Multivariate survival analysis showed that 14?3?3ζwas inde?pendent prognostic indicator for resected stage Ⅲ lung adenocarcinoma patients(P<0?05). Conclusion The expressions of 14?3?3ζand E?cadherin shows a good correlation in lung adenocarcinoma tissues. Detecting the expressions of these two proteins may give a clue on prognosis in patients with stageⅢ lung adenocarcinoma.