CAJ | 학술논문

目的：探讨哮喘发病中驱动蛋白－1（Kinesin－1）在哮喘小鼠C7～T5节段脊髓后角内的表达变化。方法 BALB／c小鼠20只，按随机数字表法均分为正常对照组和哮喘组，利用AniRes2005肺功能仪测小鼠气道阻力，免疫荧光方法和 Western 印迹方法检测各组小鼠 C7～T5节段脊髓后角内Kinesin－1的表达变化。结果哮喘组小鼠吸气阻力和呼气阻力明显高于正常组（P＜0．01），哮喘模型建立成功。免疫荧光结果显示哮喘组C7～T5节段脊髓后角内Kinesin－1阳性产物的平均光密度值（MOD）显著高于正常对照组（P＜0．01），Western印迹方法检测也得到了相同的结果。结论哮喘小鼠C7～T5节段脊髓后角内Kinesin－1的表达升高。
목적：탐토효천발병중구동단백－1（Kinesin－1）재효천소서C7～T5절단척수후각내적표체변화。방법 BALB／c소서20지，안수궤수자표법균분위정상대조조화효천조，이용AniRes2005폐공능의측소서기도조력，면역형광방법화 Western 인적방법검측각조소서 C7～T5절단척수후각내Kinesin－1적표체변화。결과효천조소서흡기조력화호기조력명현고우정상조（P＜0．01），효천모형건립성공。면역형광결과현시효천조C7～T5절단척수후각내Kinesin－1양성산물적평균광밀도치（MOD）현저고우정상대조조（P＜0．01），Western인적방법검측야득도료상동적결과。결론효천소서C7～T5절단척수후각내Kinesin－1적표체승고。
Objective To study the expression of Kinesin-1 in the spinal dorsal horn of the asthmatic mice .Methods 20 BALB/c mice were randomly divided into normal control and asthmatic groups .The airway resistance of mice was measured with AniRes 2005 pulmo-nary function meter;The expression of Kinesin-1 was observed by immunofluorescence and Western blot .Results Inspiratory resistance and expiratory resistance in the asthmatic mice were obviously higher than those of normal control mice , the asthmatic model was successfully con-structed.The mean optic density(MOD) of Kinesin-1 positive product in the C7~T5 spinal dorsal horn was increased significantly in the asthmatic mice than that in normal control by means of immunofluorescence (P<0.01), and the same result was obtained by Western blot method.Conclusions The expressions of Kinesin-1 is elevated in the C7~T5 spinal dorsal horn of the asthmatic mice .