CAJ | 학술논문

目的：探讨缝隙连接蛋白－43（Cx－43）在膀胱出口部分梗阻大鼠逼尿肌细胞膜中表达的变化。方法33只Wistar大鼠分为模型组和假手术组，两组大鼠分别于术后2、4和8 w处死，同时进行膀胱内测压、缝隙连接数量、形态变化及Cx－43的检测。结果在模型组中缝隙连接的数量显著降低，并且术后4 wCx－43在模型组大鼠逼尿肌细胞膜上的表达显著降低。模型组大鼠逼尿肌细胞膜上的缝隙连接数量也明显减少。结论正常的排尿过程是通过逼尿肌细胞间的缝隙连接直接作用的结果。缝隙连接介导的细胞间通讯功能障碍可能是导致排尿功能障碍的原因。
목적：탐토봉극련접단백－43（Cx－43）재방광출구부분경조대서핍뇨기세포막중표체적변화。방법33지Wistar대서분위모형조화가수술조，량조대서분별우술후2、4화8 w처사，동시진행방광내측압、봉극련접수량、형태변화급Cx－43적검측。결과재모형조중봉극련접적수량현저강저，병차술후4 wCx－43재모형조대서핍뇨기세포막상적표체현저강저。모형조대서핍뇨기세포막상적봉극련접수량야명현감소。결론정상적배뇨과정시통과핍뇨기세포간적봉극련접직접작용적결과。봉극련접개도적세포간통신공능장애가능시도치배뇨공능장애적원인。
Objective To investigate alterations in gap junctional protein ,connexin-43 (Cx-43),in the rat detrusor muscle with par-tial bladder outlet obstruction (P-BOO).Methods 33 female Wistar rats (10 weeks old) were divided into model and sham groups and were killed at 2,4,and 8 weeks after surgery .Cystometric investigation ,the alteration of gap junction ,and Cx-43 protein expression ,which compose the gap junction,were examined.Results The number of gap junctions was decreased in the P-BOO rat bladder.Furthermore,de-creased cellular membrane expression of Cx-43 proteins was detected in rat detrusor muscle cells more than 4 weeks after surgery .The gap junctions of the detrusor muscle cell membranes were significantly fewer in number in the P-BOO rats with no detrusor contractions .Conclu-sions The normal signals that contribute to voiding function could be transported directly through the gap junctions .Voiding dysfunction may be caused by the disruption of gap junctional intercellular communication .