CAJ | 학술논문

目的：miRNA能通过转录后调节靶基因的表达量，并在致癌过程中发挥抑癌或促癌作用。研究发现miR-30家族在人类肿瘤中起着重要作用，并参与维持干细胞特性的上皮细胞间质化过程，本研究主要识别miR-30家族与肝细胞肝癌的关系。方法：应用qRT-PCR实验方法检测93例肝细胞肝癌患者癌组织和癌旁正常组织中miR-30c，miR-30b和miR-30e表达水平，另对121例肝细胞肝癌患者癌组织进行miR-30家族的表达及CD90免疫组织化学表达检测，分析miR-30家族与CD90在肝细胞肝癌中的相关性。结果：本研究发现miR-30c（P<0.001）、miR-30b（P=0.004）和miR-30e（P<0.001）与癌旁正常组织相比，癌组织中表达显著下调。CD90蛋白在癌组织中的表达水平显著高于癌旁正常组织（P=0.007）。MiR-30c（P=0.032）和miR-30e（P=0.015）在CD90阴性表达的患者中表达水平显著高于CD90阳性表达的患者。结论：miR-30家族在肝细胞肝癌中可作为抑癌miRNA，并通过调节CD90蛋白来发挥这一作用。
목적：miRNA능통과전록후조절파기인적표체량，병재치암과정중발휘억암혹촉암작용。연구발현miR-30가족재인류종류중기착중요작용，병삼여유지간세포특성적상피세포간질화과정，본연구주요식별miR-30가족여간세포간암적관계。방법：응용qRT-PCR실험방법검측93례간세포간암환자암조직화암방정상조직중miR-30c，miR-30b화miR-30e표체수평，령대121례간세포간암환자암조직진행miR-30가족적표체급CD90면역조직화학표체검측，분석miR-30가족여CD90재간세포간암중적상관성。결과：본연구발현miR-30c（P<0.001）、miR-30b（P=0.004）화miR-30e（P<0.001）여암방정상조직상비，암조직중표체현저하조。CD90단백재암조직중적표체수평현저고우암방정상조직（P=0.007）。MiR-30c（P=0.032）화miR-30e（P=0.015）재CD90음성표체적환자중표체수평현저고우CD90양성표체적환자。결론：miR-30가족재간세포간암중가작위억암miRNA，병통과조절CD90단백래발휘저일작용。
Objective: miRNA can post-transcriptionally regulate the expression of target genes and act as oncogenes or tumor suppressor genes in tumorigenesis. Emerging evidence demonstrates that the miR-30 family may perform an important function in hu-man cancers and can regulate epithelial-mesenchymal transition, which has a critical role in cancer stem cells. This research was con-ducted to identify the possible association between the miR-30 family and hepatocellular carcinoma (HCC). Methods:The expression of miR-30c, miR-30b, and miR-30e in 93 tumor tissues and adjacent tissues were measured by real-time reverse transcription PCR. Fur-thermore, 121 tumor tissues from an independent cohort were selected to measure the expression level of miR-30 family and CD90 by immunohistochemistry. The relationship between miR-30 family and CD90 protein expression was analyzed. Results:The expression levels of miR-30c (P<0.001), miR-30b (P=0.004), and miR-30e (P<0.001) were significantly decreased in tumor tissues compared with adjacent tissues, and the expression level of CD90 in tumor tissues was significantly higher than that in adjacent normal tissues (P=0.007). In addition, the expression levels of miR-30c (P=0.032) and miR-30e (P=0.015) were significantly high in negative staining for CD90 when compared with positive staining for CD90. Conclusion:Taken together, we suggest that the miR-30 family may act as a tu-mor suppressor in HCC development and may modulate CD90 protein expression.