CAJ | 학술논문

目的：观察硒化壳聚糖对体外培养慢性粒细胞多药耐药白血病细胞株 K562／阿霉素（ ADM）细胞生物学行为的影响。方法硒化壳聚糖作用于体外培养的K562／ADM细胞12、24 h，应用流式细胞法检测细胞凋亡，软件拟合计算细胞周期；应用免疫印迹法检测P－糖蛋白（ P－gp）的表达；应用RT－PCR法检测MDR－1 mRNA水平。结果硒化壳聚糖能够明显增强 ADM对 K562／ADM细胞的诱导凋亡作用，阻滞细胞周期于 G1期（P＜0．05，P＜0．01），下调P－gp表达和MDR－1 mRNA水平（P＜0．05，P＜0．01），硒化壳聚糖浓度越高，作用效果越显著。结论硒化壳聚糖能够通过下调MDR－1基因和P－gp表达，阻滞细胞周期于G1期来诱导细胞凋亡，进而对体外培养的K562／ADM细胞耐药生物学行为产生逆转。
목적：관찰서화각취당대체외배양만성립세포다약내약백혈병세포주 K562／아매소（ ADM）세포생물학행위적영향。방법서화각취당작용우체외배양적K562／ADM세포12、24 h，응용류식세포법검측세포조망，연건의합계산세포주기；응용면역인적법검측P－당단백（ P－gp）적표체；응용RT－PCR법검측MDR－1 mRNA수평。결과서화각취당능구명현증강 ADM대 K562／ADM세포적유도조망작용，조체세포주기우 G1기（P＜0．05，P＜0．01），하조P－gp표체화MDR－1 mRNA수평（P＜0．05，P＜0．01），서화각취당농도월고，작용효과월현저。결론서화각취당능구통과하조MDR－1기인화P－gp표체，조체세포주기우G1기래유도세포조망，진이대체외배양적K562／ADM세포내약생물학행위산생역전。
Objective To investigate the effect of Selenium chiston (Sc) on biological behaviour of human leukemia multidrug re-sistance cell line K562/ADM in vitro.Methods After treated with Sc for 12,24 h,the apoptosis rate and cell phase in K 562/ADM were ex-amined by flow cytometry.Expression of P glycoprotein ( P-gp) in K562/ADM was determined by Western blot and transcription level of MDR-1 gene was detected by semi-quantitative RT-PCR.Results Sc could obviously enhance the inducing effect of ADM on apoptosis of K562/ADM cell and block cell cycle into G1 phase(P<0.05,P<0.01),down-regulate the P-gp and MDR-1 mRNA level in K562/ADM cell ( P<0.05 ,P<0.01 ) in a dose and time dependent manner .Conclusions Sc could change the biological behaviour of K 562/ADM cell in vitro through enhancing cell apoptosis ,blocking cell cycle in G1 phase,inhibiting the P-gp and MDR-1 gene expression.