CAJ | 학술논문

目的：研究左、右归丸含药血清经转化生长因子（ TGF）－β1／Smad4信号途径对骨髓间充质干细胞（ BMSCs）成骨诱导的影响。方法以左归丸、右归丸水煎液和阳性对照药补佳乐、蒸馏水给SD大鼠灌胃后取血制备含药血清（含有10％含药血清的诱导剂），与诱导剂组共5种处理因素对BMSCs进行干预，选用Western印迹法检测碱性磷酸酶（ ALP）蛋白的表达，用免疫组化 SP法检测 TGF－β1和 Smad4蛋白的表达。结果左、右归丸与空白组和诱导剂组比可显著促进ALP蛋白的表达，增强TGF－β1和Smad4蛋白的表达（P＜0．01），左归丸组与右归丸组比较可上调ALP、Smad4蛋白的表达（P＜0．01）。结论左归丸与右归丸可能通过增强ALP、TGF－β1、Smad4蛋白的表达，影响 TGF－β1／Smad4信号途径，从而协同诱导剂促进BMSCs的成骨诱导，并且左归丸较右归丸促进作用更明显。
목적：연구좌、우귀환함약혈청경전화생장인자（ TGF）－β1／Smad4신호도경대골수간충질간세포（ BMSCs）성골유도적영향。방법이좌귀환、우귀환수전액화양성대조약보가악、증류수급SD대서관위후취혈제비함약혈청（함유10％함약혈청적유도제），여유도제조공5충처리인소대BMSCs진행간예，선용Western인적법검측감성린산매（ ALP）단백적표체，용면역조화 SP법검측 TGF－β1화 Smad4단백적표체。결과좌、우귀환여공백조화유도제조비가현저촉진ALP단백적표체，증강TGF－β1화Smad4단백적표체（P＜0．01），좌귀환조여우귀환조비교가상조ALP、Smad4단백적표체（P＜0．01）。결론좌귀환여우귀환가능통과증강ALP、TGF－β1、Smad4단백적표체，영향 TGF－β1／Smad4신호도경，종이협동유도제촉진BMSCs적성골유도，병차좌귀환교우귀환촉진작용경명현。
Objective To observe the effects of serum containing ZuoGuiWan and Youguiwan on the osteogenic induction in bone marrow mesenchymal stem cells ( BMSCs) through transforming growth factor ( TGF)-β1/Smad signaling pathway .Methods Containing se-rum of Zuoguiwan,Youguiwan,positive control drug (Estradiol Valerate,BJL)and distilled water were prepared to separately add inducer , with inducer group,simultaneously intervene the BMSCs.The expression of alkaline phosphatase (ALP) protein was determined by Western blot,the expression of TGF-β1and Smad4 protein were tested by immunohistochemical streptavidin-perosidase method.Results Compared with that of control and inducer groups ,Zuiguiwan and Youguiwan remarkablely promoted the expression of ALP protein ,upregulated the ex-pressions of TGF-β1 and Smad4 protein (P<0.05).Compared with Youguiwan group ,Zuoguiwan group enhanced the expressions of ALP and Smad4 protein(P<0.05).Conclusions Through ALP enhancement the expressions of TGF-β1 and Smad4 protein,Zuiguiwan and Yougui-wan could effect TGF-β1/Smad4 signaling pathways ,thus cooperate with inducer on the osteogenic induction in BMSCs .