中国老年学杂志
中國老年學雜誌
중국노년학잡지
CHINESE JOURNAL OF GERONTOLOGY
2014年
22期
6379-6381
,共3页
胡小令%桂婷%黄涛波%吕诚%李耀斌%薛国勇%温蔚%石嘉庆
鬍小令%桂婷%黃濤波%呂誠%李耀斌%薛國勇%溫蔚%石嘉慶
호소령%계정%황도파%려성%리요빈%설국용%온위%석가경
雷公藤内酯醇%β淀粉样蛋白%阿尔茨海默病%诱导型一氧化氮合酶%突触
雷公籐內酯醇%β澱粉樣蛋白%阿爾茨海默病%誘導型一氧化氮閤酶%突觸
뢰공등내지순%β정분양단백%아이자해묵병%유도형일양화담합매%돌촉
Triptolide%Beta-amyloid protein%Alzheimer's disease%Inducible nitric oxide synthase%Synapse
目的:观察应用β淀粉样蛋白( Aβ)后大鼠海马诱导型一氧化氮合酶( iNOS)的表达和突触超微结构的变化及雷公藤内酯醇对其的影响。方法21只SD雄性大鼠等分成对照组、模型组、用药组。在大鼠左侧海马定向注射凝聚态 Aβ1~40作为模型组,注射等量生理盐水设为对照组,大鼠在海马注射凝聚态Aβ1~40后腹腔注射雷公藤内酯醇为用药组。应用免疫组织化学方法检测各组大鼠海马 iNOS的表达;应用透射电镜观察各组大鼠海马突触超微结构的变化。结果免疫组织化学染色显示,模型组大鼠海马iNOS阳性细胞数和平均光密度明显高于对照组(P<0.01),用药组大鼠海马iNOS阳性细胞平均光密度较模型组明显降低(P<0.05)。透射电镜结果显示,模型组大鼠海马神经毡内突触和突触小泡数量较对照组减少,突触后致密物厚度变薄;用药组大鼠海马神经毡内突触和突触小泡数量较模型组增多,突触后致密物增厚。结论雷公藤内酯醇可以抑制Aβ诱导的大鼠海马iNOS的表达,对突触超微结构的损伤有一定的改善作用。
目的:觀察應用β澱粉樣蛋白( Aβ)後大鼠海馬誘導型一氧化氮閤酶( iNOS)的錶達和突觸超微結構的變化及雷公籐內酯醇對其的影響。方法21隻SD雄性大鼠等分成對照組、模型組、用藥組。在大鼠左側海馬定嚮註射凝聚態 Aβ1~40作為模型組,註射等量生理鹽水設為對照組,大鼠在海馬註射凝聚態Aβ1~40後腹腔註射雷公籐內酯醇為用藥組。應用免疫組織化學方法檢測各組大鼠海馬 iNOS的錶達;應用透射電鏡觀察各組大鼠海馬突觸超微結構的變化。結果免疫組織化學染色顯示,模型組大鼠海馬iNOS暘性細胞數和平均光密度明顯高于對照組(P<0.01),用藥組大鼠海馬iNOS暘性細胞平均光密度較模型組明顯降低(P<0.05)。透射電鏡結果顯示,模型組大鼠海馬神經氈內突觸和突觸小泡數量較對照組減少,突觸後緻密物厚度變薄;用藥組大鼠海馬神經氈內突觸和突觸小泡數量較模型組增多,突觸後緻密物增厚。結論雷公籐內酯醇可以抑製Aβ誘導的大鼠海馬iNOS的錶達,對突觸超微結構的損傷有一定的改善作用。
목적:관찰응용β정분양단백( Aβ)후대서해마유도형일양화담합매( iNOS)적표체화돌촉초미결구적변화급뢰공등내지순대기적영향。방법21지SD웅성대서등분성대조조、모형조、용약조。재대서좌측해마정향주사응취태 Aβ1~40작위모형조,주사등량생리염수설위대조조,대서재해마주사응취태Aβ1~40후복강주사뢰공등내지순위용약조。응용면역조직화학방법검측각조대서해마 iNOS적표체;응용투사전경관찰각조대서해마돌촉초미결구적변화。결과면역조직화학염색현시,모형조대서해마iNOS양성세포수화평균광밀도명현고우대조조(P<0.01),용약조대서해마iNOS양성세포평균광밀도교모형조명현강저(P<0.05)。투사전경결과현시,모형조대서해마신경전내돌촉화돌촉소포수량교대조조감소,돌촉후치밀물후도변박;용약조대서해마신경전내돌촉화돌촉소포수량교모형조증다,돌촉후치밀물증후。결론뢰공등내지순가이억제Aβ유도적대서해마iNOS적표체,대돌촉초미결구적손상유일정적개선작용。
Objective To explore the effects of triptolide on the expression of inducible nitric oxide synthase ( iNOS) and synaptic ultrastructure in hippocampus of model rats with Alzheimer's disease (AD).Methods Twenty-one male SD rats were equally divided into control ,AD model and triptolide-treated groups .The AD model group were made with unilateral microinjection of aggregated beta-amyloid pro-tein( Aβ) 1~40 into hippocampus in rats and the control group rats were injected with normal saline in the same way .The triptolide-treated group rats were administered triptolide intraperitoneally after microinjection of aggregated Aβ1~40 into hippocampus .The iNOS expression and synaptic ultrastructure in hippocampus of each group were assayed by immunohistochemical staining and transmission electron microscope . Results Immunohistochemically ,the cell number and average optical density of iNOS positive staining in the AD model group were higher than those of control group (P<0.01),the average optical density of iNOS positive staining in the triptolide-treated group were lower than that of AD model group(P<0.05).The number of synapses and synaptic vesicles and the thickness of postsynaptic density of hippocampal neuro -pil in the AD model group were lower than those of control group .The number of synapses and synaptic vesicles and the thickness of postsyn -aptic density of hippocampal neuropil in the triptolide-treated group were higher than those of AD model group .Conclusions Triptolide could inhibit iNOS expression and alleviate the degeneration of synapses in hippocampus of model rats with AD .