CAJ | 학술논문

为优化双水相法提取澳洲茶树中黄酮类化合物的条件，以澳洲茶树叶的总黄酮提取率为评价指标，分别对双水相的溶剂饱和程度、乙醇浓度、料液比、提取时间、提取温度对黄酮提取率影响进行了探究。结果表明，双水相提取优化条件为溶剂饱和度磷酸二氢钾∶乙醇1∶2（w/v），70%（v/v）乙醇，料液比1∶20（w/v），提取时间为90min，提取温度90℃。提取率最高6.74%。双水相提取澳洲茶树黄酮提取率较高，且分相时间短，不存在有机溶剂残留，易于工艺放大和连续操作，是一种理想的提取方法。
위우화쌍수상법제취오주다수중황동류화합물적조건，이오주다수협적총황동제취솔위평개지표，분별대쌍수상적용제포화정도、을순농도、료액비、제취시간、제취온도대황동제취솔영향진행료탐구。결과표명，쌍수상제취우화조건위용제포화도린산이경갑∶을순1∶2（w/v），70%（v/v）을순，료액비1∶20（w/v），제취시간위90min，제취온도90℃。제취솔최고6.74%。쌍수상제취오주다수황동제취솔교고，차분상시간단，불존재유궤용제잔류，역우공예방대화련속조작，시일충이상적제취방법。
The study was based on the evaluation index of flavonoids from melaleuca ahemifolia Extraction efficiency,to optimizate method of Aqueous two-phase extraction of flavonoid compounds in Australia tea tree. The main research content including solvent saturated degree,ethanol concentration,solid-liquid ratio, extraction time,extraction temperature effect on flavonoids extraction yield of aqueous two-phase. The result showed that the conditions were ethanol 1∶2(w/v),70%(v/v)ethanol,material liquid than 1∶20(w/v),the ex?traction time was 90 min,extraction temperature 90℃,extraction efficiency rached to 6.74%. The rate of fla?vonoids is high by the method of aqueous two-phase,and the phase separation time is short,there is no or?ganic solvent residue,easy to process scale and continuous operation,is an ideal extraction method.