浙江林业科技
浙江林業科技
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JOURNAL OF ZHEJIANG FORESTRY SCIENCE AND TECHNOLOGY
2014年
5期
16-20
,共5页
程俊文%侯寒黎%邹景泉%贺亮%李肖娟
程俊文%侯寒黎%鄒景泉%賀亮%李肖娟
정준문%후한려%추경천%하량%리초연
桑黄%发酵%胞外多糖%Box-Behnken%响应面
桑黃%髮酵%胞外多糖%Box-Behnken%響應麵
상황%발효%포외다당%Box-Behnken%향응면
Phellinus igniarius%fermentation%extracellular polysaccharide%Box-Behnken%response surface methodology (RSM)
在单因素试验的基础上，通过Box-Benhnken组合设计和响应曲面分析法优化桑黄（Phellinus igniarius）液体发酵产胞外多糖的发酵工艺条件，结果表明：桑黄胞外多糖的最佳液体发酵培养条件为葡萄糖15.82 g/L，玉米粉10.51 g/L，豆饼粉9.55 g/L；此条件下的验证试验表明，此时胞外多糖的得率为461.72 mg/L，与理论值基本吻合。
在單因素試驗的基礎上，通過Box-Benhnken組閤設計和響應麯麵分析法優化桑黃（Phellinus igniarius）液體髮酵產胞外多糖的髮酵工藝條件，結果錶明：桑黃胞外多糖的最佳液體髮酵培養條件為葡萄糖15.82 g/L，玉米粉10.51 g/L，豆餅粉9.55 g/L；此條件下的驗證試驗錶明，此時胞外多糖的得率為461.72 mg/L，與理論值基本吻閤。
재단인소시험적기출상，통과Box-Benhnken조합설계화향응곡면분석법우화상황（Phellinus igniarius）액체발효산포외다당적발효공예조건，결과표명：상황포외다당적최가액체발효배양조건위포도당15.82 g/L，옥미분10.51 g/L，두병분9.55 g/L；차조건하적험증시험표명，차시포외다당적득솔위461.72 mg/L，여이론치기본문합。
Analysis of three-level Box-Behnken factorial design with response surface methodology (RSM) was conducted on fermentation technology for extracellular polysaccharides (EPS) production from Phellinus igniarius based on single factor experiment. The result showed that the optimal fermentation conditions were as follows: glucose 15.82 g/L, corn meal 10.51 g/L and soybean cake powder 9.55 g/L. Verification test demonstrated that the average yield of EPS was 461.72 mg/L, similar to theoretical value of 476.99 mg/L.
