CAJ | 학술논문

采用高效液相色谱法(HPLC)测定刺芹侧耳(Pleurotus eryngii )基质菌丝及其生长发育过程中海藻糖含量，总结其变化规律以及温度对海藻糖含量的影响。结果表明：未接种时培养料中海藻糖含量极低，为0.0094%，带料菌丝检测结果为：菌丝体阶段，海藻糖的含量均低于0.12%，随着培养天数的增加，海藻糖的含量逐渐升高；转为生殖生长以后继续升高，在原基形成时达到峰值，含量为0.14%，进入生殖生长迅速下降；菇蕾期后海藻糖含量基本保持稳定，直至采收结束，海藻糖的含量再次降低至0.05%。不同培养温度下，带料菌丝中海藻糖的含量随温度的升高逐渐增大，但温度过高时，菌丝死亡，海藻糖含量急剧下降至0.0091%。
채용고효액상색보법(HPLC)측정자근측이(Pleurotus eryngii )기질균사급기생장발육과정중해조당함량，총결기변화규률이급온도대해조당함량적영향。결과표명：미접충시배양료중해조당함량겁저，위0.0094%，대료균사검측결과위：균사체계단，해조당적함량균저우0.12%，수착배양천수적증가，해조당적함량축점승고；전위생식생장이후계속승고，재원기형성시체도봉치，함량위0.14%，진입생식생장신속하강；고뢰기후해조당함량기본보지은정，직지채수결속，해조당적함량재차강저지0.05%。불동배양온도하，대료균사중해조당적함량수온도적승고축점증대，단온도과고시，균사사망，해조당함량급극하강지0.0091%。
Trehalose levels in combined fungal mycelium/growth substrate and fruit bodies of Pleurotuseryngii were determined by high performance liquid chromatography.Trehalose levels in the cultivation substrate were relatively low (0.0094%)prior to inoculation;relatively low levels were also recorded in combined mycelium/substrate during the early mycelial growth phase (0-7 d)but gradually increased with increased cultivation time.Peak levels (0.14%)were observed at the time of primordium formation (42 d).Levels then decreased (to 0.05%)during the period of fruit body development (42-55 d).Combined mycelium/substrate trehalose levels increased slightly with increased growth temperature,with peak values (0.16%-0.17%)occurring at 31 ℃,but a higher temperature(37 ℃)led to hyphal death and much lower trehalose levels (0.0091%).