CAJ | 학술논문

基于草菇(Volvariella volvacea )基因组和转录组数据,通过生物信息学的方法对草菇α-淀粉酶基因进行基本理化性质、内含子和外显子结构、信号肽、亚细胞定位和功能位点的预测与分析,并构建系统发育树。结果表明：编码草菇α-淀粉酶的基因有5个,分别为 GME 2151、GME 6695、GME 9075、GME 10698和GME 10705；5个基因编码的蛋白相对分子量介于38.8~64.6 kD 之间,磷酸化以 Ser 位点为主,大都存在信号肽,亚细胞定位在细胞外,保守结构域和空间结构相似度较高。和其它的担子菌一样,草菇α-淀粉酶可以分为两类：GME9075和 GME10698归为α-淀粉酶Ⅰ类,GME2151、GME6695和 GME10705属于α-淀粉酶Ⅱ类。
기우초고(Volvariella volvacea )기인조화전록조수거,통과생물신식학적방법대초고α-정분매기인진행기본이화성질、내함자화외현자결구、신호태、아세포정위화공능위점적예측여분석,병구건계통발육수。결과표명：편마초고α-정분매적기인유5개,분별위 GME 2151、GME 6695、GME 9075、GME 10698화GME 10705；5개기인편마적단백상대분자량개우38.8~64.6 kD 지간,린산화이 Ser 위점위주,대도존재신호태,아세포정위재세포외,보수결구역화공간결구상사도교고。화기타적담자균일양,초고α-정분매가이분위량류：GME9075화 GME10698귀위α-정분매Ⅰ류,GME2151、GME6695화 GME10705속우α-정분매Ⅱ류。
Five genes (GME 2151、GME 6695、GME 9075、GME 1069 and GME 10705 ) were identified as encoding α-amylases in Volvariella volvacea , the molecular weights of which varied from 38.8 kD to 64.4 kD.Bioinformatic methods based on genome and transcriptome sequences have been used to analyze gene intron:exon distribution patterns and the physicochemical properties of the encoded α-amylases.Signal peptides,sub-cellular localization patterns and functional sites of the α-amylases were predicted,and a phylogenetic tree was constructed based onα-amylases from different fungi.Serine phosphorylation sites were the primary sites of amylase protein phosphorylation. The amylases contained signal peptides, transmembrane helices,conserved amino acid residues,similar three dimensional structures of amylase,and were located both intra-and extracellularly.Analysis of the phylogenetic tree revealed that the α-amylases were of two types:GME9075 and GME10698 belonged to α-amylase type I,and GME2151,GME6695 and GME10705 α-amylase type II. This is consistent with the classification of amylases from other basidiomycetes.Our data provide useful information relating to matrix degradation by the mycelium of V .volvacea and other macro-basidiomycetes.