CAJ | 학술논문

目的：通过建立感染性休克大鼠模型，探索电刺激迷走神经对感染性休克大鼠血浆中钙结合蛋白 S100A8表达水平的影响。方法：雄性成年 SD 大鼠30只，采用盲肠结扎穿孔法（CLP）建立感染性休克模型，将30只大鼠随机分为6组：①假电针正常组；②电针正常组；③假电针 CLP 组；④电针 CLP 组；⑤迷切假电针 CLP 组；⑥迷切电针 CLP 组。③～⑥组动物均行颈总动脉置管，连续监测平均动脉压；采用 ELISA 法检测各组大鼠的血浆钙结合蛋白 S100A8。结果：CLP 组手术后平均动脉压呈进行性下降，手术后12 h 时血浆钙结合蛋白 S100A8水平显著升高（P ＜0．01）；与 CLP 组比较，电刺激组动物平均动脉压下降程度较低（P ＜0．01）；迷切电针 CLP 组与迷切假电针 CLP 组相比，大鼠平均动脉压下降程度较低，血浆钙结合蛋白S100A8水平显著下降（P ＜0．01）。结论：电刺激迷走神经可以缓解盲肠结扎穿孔导致的大鼠感染性休克所引发的进行性动脉压下降，降低血浆钙结合蛋白 S100A8水平，对于治疗休克有辅助作用。
목적：통과건립감염성휴극대서모형，탐색전자격미주신경대감염성휴극대서혈장중개결합단백 S100A8표체수평적영향。방법：웅성성년 SD 대서30지，채용맹장결찰천공법（CLP）건립감염성휴극모형，장30지대서수궤분위6조：①가전침정상조；②전침정상조；③가전침 CLP 조；④전침 CLP 조；⑤미절가전침 CLP 조；⑥미절전침 CLP 조。③～⑥조동물균행경총동맥치관，련속감측평균동맥압；채용 ELISA 법검측각조대서적혈장개결합단백 S100A8。결과：CLP 조수술후평균동맥압정진행성하강，수술후12 h 시혈장개결합단백 S100A8수평현저승고（P ＜0．01）；여 CLP 조비교，전자격조동물평균동맥압하강정도교저（P ＜0．01）；미절전침 CLP 조여미절가전침 CLP 조상비，대 서 평 균 동 맥 압 하 강 정도교저，혈장개결합단백S100A8수평현저하강（P ＜0．01）。결론：전자격미주신경가이완해맹장결찰천공도치적대서감염성휴극소인발적진행성동맥압하강，강저혈장개결합단백 S100A8수평，대우치료휴극유보조작용。
Objective:To establish septic shock rat model and to investigate the effect of electrical stimulation of vagus nerve on the expression level of plasma calcium-binding protein S100A8 in septic shock rats.Methods:Thirty male adult rats were per-formed cecal ligation and puncture (CLP)to establish septic shock model and then randomly divided into six group:Group I, sham electric acupuncture and normal;Group II,electric acupuncture and normal;Group III,sham electric acupuncture and CLP;Group IV,electric acupuncture and CLP;Group V,vagotomy,sham electric acupuncture and CLP;Group VI,vagoto-my,electric acupuncture and CLP.In Group III,IV,V and VI,indwelling catheter was placed in the common carotid artery for monitoring mean arterial blood pressure (MAP)continuously.Plasma calcium-binding protein S100A8 was determined by ELISA.Results:In groups with CLP,MAP gradually decreased,and serum S100A8 level at postoperative 12 h significantly increased (P <0.01).Compared to that in groups with CLP,the decreased extent of MAP reduced in rats treated with electri-cal stimulation (P <0.01).Moreover,the decreased extent of MAP reduced and the level of plasma calcium-binding protein S100A8 significantly decreased in Group VI,compared to that in Group V (P <0.01).Conclusions:The electrical stimulation of vagus nerve can alleviate gradual decrease of MAP which was caused by CLP induced septic shock in rats and reduce the level of plasma calcium-binding protein S100A8,so that it contributes to anti-shock treatment as supplementary role.